【摘要】 为探讨中国美利奴羊(新疆军垦型)不同单倍型个体感染细粒棘球绦虫的免疫应答变化。将8只抗性单倍型绵羊设为抗性组,8只非抗性单倍型绵羊设为对照组,在相同的饲养条件下进行人工感染攻虫。分别在攻虫前7d(-7d)、攻虫当天(0d)、攻虫后第7、21、30、60天用流式细胞仪分析外周血中CD4+T、CD8+T、B淋巴细胞亚群的变化,同时用ELISA方法定量分析血清中IgG、IgM、IgE、IFN-γ水平并进行粒细胞计数。结果表明,抗性组绵羊的发病率显著低于对照组(P=0.019);CD4+T细胞2组之间差异不显著(P>0.05),CD8+T细胞在攻虫后第7天抗性组显著高于对照组(P<0.05);IgG水平在攻虫后第7天2组均显著高于攻虫前7d(P<0.05)。攻虫后第30天抗性组IgM和IFN-γ水平均显著高于对照组(P<0.05)。抗性组的白细胞、淋巴细胞分别在攻虫后第7和21天显著高于对照组(P<0.05),嗜中性粒细胞和总蛋白在攻虫后第21天抗性组极显著低于对照组(P<0.01)。结果显示,抗性单倍型绵羊对细粒棘球绦虫的抵抗力显著高于非抗性绵羊;抗性组绵羊体内的CD8+T细胞、IgM、IFN-γ和白细胞、淋巴细胞水平在攻虫后不同时期显著高于非抗性组绵羊。更多还原

【Abstract】 The aim of this study was to analyze the immunological response to Echinococcus granulosus in Chinese Merino(Xinjiang Army Wasteland Reclamation) sheep with different MHC-DRB1/DQB1 haplotypes.16 two-year-old female healthy Chinese Merino sheep with different haplotypes were artifically infected with Echinococcus granulosus under the same raising condition,which were divided into two groups:the test group(8 resistant haplotyoe sheep) and the control group(8 nonresistant haplotype individuals).The percentage of CD4+ T,CD8+ T and B lymphocyte subpopulations in peripheral blood were analyzed by flow cytometry on 7 day before infection(-7),and on 0(the day of infection),7,21,30 and 60 day after infection,respectively.During the same period,the content of IgG,IgM,IgE,IFN-γ in the serum were quantitatively assayed by ELISA and the quantity of granulocyte were counted on Wright’s stained blood smears.The results showed that:1) the incidence of Cystic Echinococcosis in the test group was significantly lower than that in the control group(P=0.019).2) The percentage of CD4+ T cells had no significant difference between the two groups(P>0.05).The percentage of CD8+ T cells in the test group was significantly higher than that in the control group(P<0.05) at day 7.3) Serum levels of IgG in the two groups were significantly higher at day 7 when compared to day(-7)(P<0.05).Compared with the test group,significantly higher IgM and IFN-γ occurred in the control group at day 30(P<0.05).4) Leukocytes and lymphocytes in the test group were significantly more than that in the control group(P<0.05) at day 7 and 21.Neutrophils and albumin in the test group were obviously lower than that in the control group(P<0.01) at day 21.The significantly higher resistance to Echinococcus granulosus occurred in the resistant haplotype sheep,when compared with the nonresistant haplotype individuals.In addition,the levels of CD8+ T cells,IgM,IFN-γ,leukocytes and lymphocytes in the resistant haplotype sheep were significantly higher than those in nonresistant haplotype individuals during the different periods after infection.更多还原