【摘要】 旨在对山羊卵巢有腔卵泡发育中基因表达特点进行研究。本研究应用抑制消减杂交技术(SSH)对黄淮山羊卵泡期卵巢非闭锁大卵泡(6 mm)和小卵泡(4 mm)颗粒细胞内差异表达基因进行了研究,建立了消减cDNA文库,通过斑点杂交分析,从正向文库中随机挑取96个克隆进行差异表达的筛选,结果发现,其中有8个与已知功能基因高度相似,12个全新的表达序列标签(EST)。结果显示,这些基因可能影响着山羊卵泡的发育成熟和排卵。更多还原

【Abstract】 The aim of this study was to analyze the gene expression during the goat antral follicle development.The different expressed genes in granulosa cells between non-latching large follicles(LF,6 mm) and small follicles(SF,4 mm) during follicular phase of Huanghuai goat ovary were studied using suppressive subtractive hybridization(SSH),and reduction cDNA library were also established.Furthermore,96 clones randomly picked from the forward library were screened for differential expression by dot blot analysis.The results showed that eight clones had highly similar with the known genes,and twelve clones were new expressed sequence tags(ESTs).The results indicated that these genes may influence the maturation and ovulation of goat follicle.更多还原