【摘要】 本研究旨在分析DRD2基因与鸡产蛋性状的相关性,寻找可作为鸡产蛋性状的重要标记。在该基因上选取了14个多态位点,采用PCR-RFLP及PCR测序等方法在宁都三黄鸡母系644个个体中进行了鸡产蛋性状的关联分析。结果表明:位于该基因5′调控区的4个突变位点G-38560C、T-38326G、T-32751C和A-16105G与鸡的开产日龄显著或极显著(P<0.05或P<0.01)相关,位点A-16105G还与300日龄总产蛋数显著相关(P<0.05);由G-38560C、G-38544A、I-38463D与T-38326G这4个位点组成的单倍型块与开产日龄呈极显著相关(P=0.006 5<0.01)。研究结果提示:G-38560C、T-38326G、T-32751C和A-16105G这4个突变位点以及由G-38560C、G-38544A、I-38463D与T-38326G组成的单倍型块可作为产蛋性状标记辅助选择的有效分子标记。更多还原

【Abstract】 The purpose of this study was to analyze the associations of the DRD2 gene with chicken egg laying traits and find important markers for these traits.Fourteen polymorphic sites of this gene were selected,and the association analysis was performed in 644 chickens of Ningdu Sanhuang female line by PCR-RFLP and sequencing methods.The results showed that 4 mutation sites in the 5′regulatory region of the DRD2 gene,G-38560C,T-38326G,T-32751C and A-16105G,were significantly associated with the age of first egg(AFE)(P<0.05 or P<0.01).A-16105G was also significantly associated with total egg number at 300 days(EN300)(P< 0.05).The haplotype block consisting of G-38560C,G-38544A,I-38463D and T-38326G was significantly associated with AFE(P<0.05).It was suggested that G-38560C,T-38326G,T-32751C and A-16105G on the DRD2 gene and the haplotype block consisting of G-38560C,G-38544A,I-38463D and T-38326G could be used as effective molecular markers in marker assisted selection(MAS) for egg laying traits.更多还原