【摘要】 选择年龄、体质量相近的杂交母羊(萨福克♂×小尾寒羊♀)24只,随机分为3组,分别饲喂不同蛋白水平的日粮:中蛋白日粮(150 g.d-1)、低蛋白日粮(112.5 g.d-1)和高蛋白日粮(187.5 g.d-1)。采用半定量RT-PCR的方法,检测日粮不同蛋白水平对绵羊脂肪和肌肉中FAS基因表达的影响。结果表明:日粮的蛋白质水平影响绵羊腹部皮下脂肪、肠系膜脂肪和半腱肌组织中FAS基因的表达,随着日粮蛋白质水平的升高FAS表达量降低。FAS基因表达量在各组织间有差异,腹部皮下脂肪、肠系膜脂肪中,中蛋白组和低蛋白组间表达差异不显著(P>0.05),高蛋白组显著低于中蛋白组和低蛋白组(P<0.05);在半腱肌组织中,中蛋白组与高蛋白组和低蛋白组间均差异显著(P<0.05),高蛋白组极显著低于低蛋白组(P<0.01)。更多还原

【Abstract】 Twenty four crossbred ewes(Black-Suffolk♂× Small Tail Han sheep♀) were randomly allocated into three groups fed with different protein levels diet,medium protein(150 g·d-1),low protein(112.5 g·d-1) and high protein(187.5 g·d-1) groups,respectively.The expression level of FAS mRNA in adipose tissue and muscle of sheep were quantified by RT-PCR.The result showed that expression of FAS mRNA decreased with the increase of diet protein level in abdominal subcutaneous adipose tissue,mesentery adipose tissue and semitendinosus muscle of sheep.But the expression of FAS mRNA had tissue specificity,it was expressed in abdominal subcutaneous and mesentery adipose tissue,there was no significant difference between medium protein group and low protein group(P>0.05),but the expression of high protein group was significantly lower than that of medium protein group and low protein group(P<0.05).While in semitendinosus muscle tissue,the expression of FAS mRNA in medium protein group was significantly different from that in high protein group and low protein group(P<0.05),and the expression of FAS mRNA in high protein group was significantly higher than that in low protein group(P<0.01).更多还原