【摘要】 本试验应用免疫组织化学方法和显微图像分析技术,研究了2、16、30、44、58日龄岭南黄鸡外周器官中Ghrelin免疫反应阳性细胞的定位分布与发育性变化。结果表明,在鸡腺胃、肠、心、肝、脾、肺、肾、胰、脑垂体、肾上腺、胸腺、法氏囊中均可观察到Ghrelin免疫阳性反应。其阳性细胞类型包括:腺胃腺小管的内分泌细胞,肠道黏膜上皮和肠腺内的内分泌细胞,肠道黏膜下层和肌层间的神经丛,心内膜下层的蒲肯野纤维,肝血窦中的枯否氏细胞,脾的巨噬细胞和网状细胞,肺的巨噬细胞,肾脏肾小囊壁层的扁平上皮细胞和脏层的足细胞、球内系膜细胞和球旁复合体,胰腺的胰岛细胞,腺垂体的部分嗜酸性细胞和嗜碱性细胞,肾上腺的嗜铬细胞,胸腺上皮细胞、胸腺小体和巨噬细胞,法氏囊黏膜上皮和小结相关上皮内的内分泌细胞、囊小结的网状细胞、巨噬细胞、皮质髓质交界处的上皮细胞等。2~30日龄鸡,随着日龄的增长,各器官中的Ghrelin表达量增加,44、58日龄则有所下降。更多还原

【Abstract】 In order to investigate the distribution of ghrelin-immunopositive cells in chickens,some samples of broiler chickens aged 2,16,30,44 and 58 d were collected and studied by immunohistochemistry and microscopic image analysis.The ghrelin-immunopositive reaction was found in the glandular stomach,intestine,heart,liver,spleen,lung,kidney,pancreas,pituitary,adrenal gland,thymus,and bursa of Fabricius.The types of ghrelin-immunopositive cells(ghrelin-ic) were as follows: some endocrine cells and nerve plexes in the glandular stomach and intestine;Purkinje fibers in heart;Kupffer′s cells in liver;macrophages and reticular cells in spleen;pulmonary macrophages;podocytes,Intraglomerular mesangial cells,Juxtaglomerular cells in kidney;pancreatic islets cells in pancreas;some acidophilic cells and basophilic cells in pituitary;chromaffin cells in adrenal gland;thymic epithelial cells,thymic corpuscles and macrophages in thymus;some endocrine cells,reticular cells,macrophages and epithelial cells in bursa of Fabricius.With the chicken growth,the expressive contents of ghrelin were increased from aged 2 to 30 d,but slightly decreased at aged 44 and 58 d in various organs of chickens.更多还原