【摘要】 本研究旨在寻找ABCG2基因上与产乳性状相关的多态位点,为提高产乳性状提供理论依据。本研究采用PCR-SSCP技术分析了ABCG2基因exon9的多态性。使用SAS9.0对多态性与产乳性状之间的相关性进行方差分析。研究结果表明,在exon9中存在A→G突变,导致氨基酸由酪氨酸突变为半胱氨酸。A、B 2个等位基因的频率分别为0.86和0.14;存在3种基因型:AA、AB和BB型,基因型频率分别为:0.73、0.26、0.01;多态信息含量为0.22。方差分析结果显示,3种基因型间5种产乳性能的差异不显著。Y367C与产乳性状的相关性不明显,由于突变前后氨基酸性质发生了改变,可能会对蛋白质的结构产生一定的影响。更多还原

【Abstract】 This study was conducted to analyze the polymorphisms of ABCG2 gene in Chinese Holstein dairy and aid to provide a theoretical foundation for further research on correlation between ABCG2 gene and milk production performance.The polymorphism of exon9 of ABCG2 gene was detected by PCR-SSCP,and the SAS 9.0 was used to analyze the variance between genotypes and milk production performance.The result showed that a single nucleotide change(A/G) in exon9 resulted in the mutation from Tyrosine-367 to Cysteine(Y367C) in the ABCG2(ATP binding cassette,subfamily G,member 2) gene.There were two alleles: A and B,and their allele frequencies were 0.86 and 0.14,respectively.Three genotypes(AA,AB and BB) were found in our materials.The frequency of AA,AB and BB were 0.73,0.26 and 0.01,respectively.The PIC(Polymorphism Information Content) was 0.22.The milk production performance,individuals with BB genotype were all highest than those with AA and AB genotypes but not significant.The relationship between Y367C and milk production performance was not obviously,but the structure of ABCG2 protein may be influenced by Y367C changed.更多还原