【摘要】 [目的]克隆人CYP19基因启动子PⅠ.3(-345～-234bp)、PⅡ(-517～-278bp)和PⅠ.7(-299～+81bp)片段,构建启动子荧光素酶报告基因载体。[方法]采用生物合成PⅠ.3(-345～-234bp)、PⅡ(-517～-278bp)和PⅠ.7(-299～+81bp)区域基因片段,通过HindⅢ和KpnⅠ酶切连接到pGL3-Basic载体上。[结果]测序验证pGL3-Basic-PⅠ.3,pGL3-Basic-PⅡ和pGL3-Basic-PⅠ.7构建成功。[结论]pGL3-Basic-PⅠ.3,pGL3-Basic-PⅡ和pGL3-Basic-PⅠ.7荧光素酶报告基因载体的成功构建,为进一步研究CYP19基因组织特异性表达奠定基础。更多还原

【Abstract】 [Purpose] To construct luciferase report gene vector containing human CYP19 promotor PⅠ.3(-345--234bp),PⅡ(-517--278bp)and PⅠ.7(-299-+81bp).[Methods] PⅠ.3(-345-234bp),PⅡ(-517--278bp)and PⅠ.7(-299-+81bp) were amplified by biosynthesis,and inserted into the luciferase report gene pGL3-Basic vector with restriction endonuclease HindⅢ and Kpn Ⅰ.[Results] pGL3-Basic-PⅠ.3,pGL3-Basic-PⅡ and pGL3-Basic-PⅠ.7 were confirmed by DNA sequencing.[Conclusion] The human CYP19 promotor luciferase reporter gene vectors are constructed successfully,which establishes the base for the further research of CYP19 tissue-specific expression mechanism.更多还原