【摘要】 目的:观察奥美沙坦对大鼠移植静脉内膜重构过程中整合素β-3(integrinβ-3)黏着斑激酶(focal adhesion kinase,FAK)表达的影响。方法:建立大鼠移植静脉桥模型,将36只雄性SD大鼠被随机分成模型对照组、奥美沙坦组及生理盐水对照组,每组12只。取移植静脉切片、HE染色观察各组血管壁内膜的厚度。采用免疫组化染色法观察各组血管平滑肌细胞(SMC)增殖核抗原(PCNA)的表达。采用Western blot法检测整合素β-3和FAK的表达。结果:与模型对照组相比,奥美沙坦组内膜的厚度明显减轻(P<0.05);PCNA和FAK的表达明显降低(P<0.05)。生理盐水对照组与模型对照组相比,内膜的厚度无明显减轻;PCNA和FAK的表达无明显改变。结论:大鼠移植静脉内膜重构过程中,伴随整合素β-3/FAK信号蛋白的表达,奥美沙坦可抑制大鼠移植静脉内膜重构,此作用可能与下调FAK信号蛋白的表达有关。更多还原

【Abstract】 AIM: To observe the expression of focal adhesion kinase in rat’s autologous vein graft and its modulating effect by olmesartan(a specific type 1 angiotensin II receptor antagonist).METHODS: Autologous external jugular veins were grafted to common carotid arteries in 36 male Wistar rats.After surgery,rats were randomly assigned to three groups(12 rats in each): model control group,olmesartan group and physiological saline group.The intimal thickness in vein grafts was quantitated in H/E-stained segments.Integrin β-3 and focal adhesion kinase expression levels were assessed by Western blotting,and proliferating cell nuclear antigen(PCNA) was measured by immunohistochemistry.RESULTS: Neointimal hyperplasia was observed in model control group characterized by significant intimal thickening.The expressions of integrin β-3,FAK and PCNA were positive in model control group.Compared with that in model control group,the intimal thickness was significantly attenuated in olmesartan group(P<0.05) and the expressions of FAK and PCNA significantly reduced(P<0.05).CONCLUSION: FAK is involved in the restenosis of vein graft in rats.Olmesartan attenuates the restenosis of rat vein graft,possibly through its downregulating effect on FAK.更多还原