【摘要】 目的比较糖尿病合并结核感染诊断中四种结核分枝杆菌检测方法,包括金胺O荧光染色涂片法,BacT/ALERT3D快速培养法,噬菌体生物扩增法和荧光定量PCR法。方法收集糖尿病合并结核感染患者的痰标本,分别采用上述四种方法进行结核分枝杆菌检测。结果涂片法、快速培养法、噬菌体法和PCR法的阳性率分别为27.5%,38.3%,36.7%和39.2%。结论在糖尿病合并结核感染诊断中,与传统的涂片法和培养法相比,噬菌体生物扩增法和荧光定量PCR法检测结核分枝杆菌具有快速、敏感性高、特异性高等特点。更多还原

【Abstract】 Objective To compare four methods of detection for Mycobacterium tuberculosis in diabetes mellitus complicated by tuberculosis infection,inculding Auramine O fluorescence staining,BacT/ALERT 3D fast culture,phage biological amplification and fluorescence quantitative PCR.Methods Sputum specimens from patients of diabetes mellitus complicated by tuberculosis infection were detected by the four methods.Results The positive rates with smears test,fast culture,phage biological amplification and PCR were 27.5%,38.3%,36.7%,39.2%,respectively.Conclusion Phage biological amplification and fluorescence quantitative PCR were rapid,sensitive and specific compared to smear and culture in diagnosis of diabetes mellitus complicated by tuberculosis infection.更多还原