【摘要】 [目的]探讨枸杞多糖(LBP)对染镉大鼠肝氧化性损伤的拮抗作用。[方法]实验动物为健康雄性Wis-tar大鼠,随机分为5组,为对照组,单纯染镉组,干预1组,干预2组和干预3组,对照组灌胃0.9%NaCl,2h后腹腔注射0.9%NaCl,单纯染镉组灌胃0.9%NaCl,2h后腹腔注射氯化镉1.5mg/kg,干预1、2、3组分别灌胃LBP5mg/kg、10mg/kg、20mg/kg,2h后各组腹腔注射氯化镉1.5mg/kg,连续5d,d6取肝脏测定超氧化物歧酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽(GSH)。[结果]与对照组相比,镉染毒组的SOD活力下降,MDA含量升高,GSH-Px活力下降,GSH含量下降;干预1、2、3组肝脏SOD分别是镉染毒组的101.8%、113.2%、114.6%,MDA分别是镉染毒组的75.3%、63.8%、43.5%,GSH-PX分别是镉染毒组的111.6%、160.3%、158.8%,GSH分别是镉染毒组的115.0%、132.6%、136.1%。[结论]LBP对镉染毒致大鼠肝氧化损伤有一定的拮抗作用。更多还原

【Abstract】 [Objective] To study the antagonistic effect of LBP on hepatic oxidative damage induced by cadmium in rats. [Methods] Wistar rats were randomly divided into five groups: control group,cadmium group,interventional group 1,interventional group 2 and interventional group 3. Control group was intragastrically given with 0.9% NaCl and intraperitoneally injected with 0.9% NaCl two hours later. Cadmium group was gastric lavage with 0.9% NaCl and intraperitoneally injected with cadmium chloride(CdCl2) 1.5mg /kg two hours later. LBP of 5,10 and 20mg /kg were gastric lavage of the rats of the interventional group 1,2,3 respectively,and then were intraperitoneally injected with CdCl2 1.5mg /kg two hours later. All the groups were treated for five days. At the sixth day,liver of the rats was collected. The levels of superoxide dismutase(SOD) and malonydialdehyde(MDA) and glutatione peroxidase(GSH-Px) and glutathione(GSH) in the liver were measured. [Results] In cadmium group,the levels of superoxide dismutase(SOD) ,glutatione peroxidase(GSH-Px) and glutathione(GSH) in the liver decreased and malonydialdehyde(MDA) increased. Compared with the cadmium group,the SOD in interventional group 1,2 and 3 increased to 101.8%,113.2% and 114.6%,respectively. The MDA decreased to 75.3%,63.8% and 43.5%,respectively,the GSH-Px increased to 111.6%,160.3% and 158.8%,respectively,and the GSH increased to 115.0%,132.6% and 136.1%,respectively. [Conclusion] Pretreatment with LBP in rats could antagonize hepatotoxicity induced by cadmium to a certain extent.更多还原