【摘要】 [目的]应用多重PCR技术筛查自腹泻病人中分离的致泻大肠埃希菌,了解5种致泻大肠埃希菌的感染情况。[方法]用常规方法分离培养粪便标本中的大肠埃希菌,提取DNA,采用多重PCR扩增12对目的基因,电泳检测扩增产物,确定病原检出情况,并进行统计学分析。[结果]2007年河南省1534份标本中致泻大肠埃希菌检出率10.37%,其中EAEC(3.59%)居首位,其余依次检出EPEC(2.67%)、STEC(2.15%)、ETEC(1.63%)、EIEC(0.33%)。合并感染检出率0.78%,以ETEC合并感染居多。感染高峰在5月(19.85%),城市与农村的检出率差异无统计学意义,45～60岁组腹泻病人的检出率最高(17.00%)。[结论]应用多重PCR技术能快速鉴别粪便标本中的致泻大肠埃希菌。致泻大肠埃希菌是河南省常见的腹泻病原。更多还原

【Abstract】 [Objective] To screen five types of diarrheagenic Escherichia coli isolated from diarrheal patients with multiplex PCR technique and to clarify the detected rate of pathogens. [Methods] Escherichia coli strains were isolated from stool specimens and cultured according to the conventional method. Then DNA was extracted. Twelve templates were amplified with multiplex PCR technique. The products of amplification were analyzed by electrophoresis. The diarrheal patients with pathogenic infections were epidemiologically and statistically described. [Results] One hundred and fifty-nine (10.37%) diarrheagenic Escherichia coli isolates were identified from 1534 stool specimens of diarrheal patients in Henan in 2007. The most common pathogen was EAEC (3.59%), followed by EPEC (2.67%), STEC (2.15%), ETEC (1.63%) and EIEC (0.33%). The detected rate of mix pathogens was 0.78%, among them ETEC was the most co-infectious pathogen. The peak season of diarrheal illness with pathogenic infections appeared on May. There were no significant differences of the detected rates of pathogens between rural and urban areas. The detected rate of pathogens was highest in those aged over 45 years old and under sixty years old. [Conclusion] Diarrheagenic Escherichia coli from diarrheal patients can be identified rapidly by multiplex PCR technique. Diarrheagenic Escherichia coli is the most common pathogen in Henan Province.更多还原