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应用荧光定量PCR技术检测肠道病毒71型
THE APPLICATION OF REAL-TIME FLUORESCENT PCR FOR DETECTION OF ENTEROVIRUS TYPE 71
【摘要】 [目的]探讨实时荧光RT-PCR和逆转录聚合酶链反应(RT-PCR)检测肠道病毒EV71病毒核酸的应用。[方法]采集2008年5月深圳市龙岗区幼儿疑似肠道病毒感染者粪便和疱疹液标本,分别用实时荧光RT-PCR方法和逆转录聚合酶链反应(RT-PCR)方法对肠道病毒EV71病毒进行检测。[结果]在对16例已收集到的患者的粪便、疱疹液标本进行检测时,用实时荧光RT-PCR方法检测到8份阳性样本,阳性率为50.O%(8/16),而用逆转录聚合酶链反应(RT-PCR)方法检测到5份阳性样本,阳性率为31.3%(5/16)。[结论]实时荧光RT-PCR技术具有特异性强,灵敏度高的特点,且能在采样后数小时内检测到病毒的RNA,从而达到快速诊断的目的,因此该方法对肠道病毒的流行病学调查及治疗起重要作用。
【Abstract】 [Objective] To study on the application of real-time fluorescent RT-PCR and reverse transcription polymerase chain reaction(RT-PCR) for the detection of Enterovirus type 71 nucleotide.[Methods] Stool and herpes fluid specimens were collected from children with suspect Enterovirus infection in Longgang District of Shenzhen in May 2008.Real-time fluorescent RT-PCR and RT-PCR were applied to detect EV71.[Results] A total of 16 samples were collected from stool and herpes fluid specimens and were performed the detection of EV71.Eight of them were positive by real-time fluorescent RTPCR,the positive rate was 50.0%(8 /16),while five samples showed to be positive by RT-PCR,and the positive rate was 31.3%(5 /16).[Conclusion] Real-time fluorescent RT-PCR is a high specific and sensitive method and can detect virus RNA within several hours after specimen collections,so that it can rapidly diagnose virus DNA and plays an important role for the investigation of epidemiology and treatment.
【Key words】 Enterovirus EV71; Real-time fluorescent RT-PCR; RT-PCR;
- 【文献出处】 现代预防医学 ,Modern Preventive Medicine , 编辑部邮箱 ,2010年02期
- 【分类号】R450
- 【被引频次】4
- 【下载频次】294