【摘要】 目的探讨骨桥蛋白(OPN)相关基因在口腔癌侵袭转移中的作用。方法采用免疫组织化学SP法,检测OPN、核因子-κB p65(NF-κB p65)和基质金属蛋白酶-9(MMP-9)在58例口腔癌和10例正常口腔粘膜中的表达。结果在10例正常口腔粘膜组织中,OPN、NF-κB p65和MMP-9均无阳性表达。在32例无颈淋巴结转移的口腔癌组中OPN、NF-κB p65和MMP-9的表达率依次为40.6%、34.4%和37.5%,在26例伴有颈淋巴结转移的口腔癌组中其阳性率依次为69.2%、65.4%和84.6%,其阳性表达率在有转移的口腔癌组中高于无转移的口腔癌组,两组比较差异有统计学意义(P<0.05),而与患者性别、年龄、肿瘤大小、组织学分级均无相关性(P>0.05)。OPN和NF-κB p65的表达与NF-κB p65和MMP-9的表达均呈正相关(r1=0.57,r2=0.52,P<0.05)。结论 OPN可能通过活化NF-κB,上调转移相关分子MMP-9的表达,从而促进口腔癌细胞的侵袭转移。更多还原

【Abstract】 Objective To investigate the correlation between the expression of osteopontin(OPN) related genes and invasion and metastasis in oral squamous cell carcinoma(OSCC).Methods The expression of OPN,nuclear factor-κB p65(NF-κB p65) and matrix metalloproteinase 9(MMP-9) was detected by immunohistochemistry in 10 cases of normal oral mucosa and 58 cases of OSCC.Results OPN,NF-κB p65 and MMP-9 were not expressed in normal oral mucosa.The expression rate was 40.6%,34.4% and 37.5% respectively in 32 OSCC samples without cervical lymph node metastasis,but the rate increased to 69.2%,65.4% and 84.6% respectively in 26 OSCC samples with cervical lymph node metastases.There was significant difference in the expression rate between the group with or without cervical lymph node metastasis(P<0.05),there was no significant difference between the expression rate and gender,age at diagnosis,tumor size and histological grade(P>0.05).Correlations existed between OPN and NF-κB p65 as well as between NF-κB p65 and MMP-9(r1=0.57,r2=0.52,P<0.05).Conclusion Osteopontin overexpression is closely related to the invasion and metastasis of OSCC through MMP-9 up-regulation via activating NF-κB p65.更多还原