【摘要】 目的评价健康人口腔黏膜的组织学状况及其Ki-67和Cox-2的表达。方法采集50例健康志愿者口腔黏膜和10例存档口腔白斑标本,进行组织学观察和Ki-67与Cox-2的免疫组织化学研究。结果 50例受检健康人口腔黏膜肉眼观察均为红润、柔软;组织学观察发现其中10例为正常口腔黏膜的组织学表现,另40例表现为不同程度的黏膜增生水肿,其组织学改变与黏膜白色水肿极其相似。10例黏膜白斑表现为上皮增生,表面过度正角化。免疫组织化学观察发现,Ki-67表达在正常口腔黏膜(5.24%)与增生水肿的口腔黏膜(14.41%)和黏膜白斑(12.08%)之间,差异有显著性(P<0.05);而增生水肿的口腔黏膜和白斑之间,差异无显著性(P>0.05)。Cox-2表达从正常口腔黏膜(0)到增生水肿的口腔黏膜(55.0%)和白斑(70.0%)之间,逐渐增高。正常口腔黏膜Cox-2阴性表达与增生水肿的口腔黏膜和白斑之间,差别有显著性(P<0.05);而增生水肿的口腔黏膜和白斑之间,差别无显著性(P>0.05)。结论本研究中,约80%的受检健康人口腔黏膜存在组织病理学改变。更多还原

【Abstract】 Objective To evaluate the expressions of Ki-67 and Cox-2 in relation to histological changes of the oral mucosa of healthy people and the potential possibility of incidence of oral mucosa disease.Methods Fifty samples of oral mucosa from 50 volunteers of healthy people and 10 samples of leukoplakia from clinical patients were enrolled in this study. Tissue sections were prepared from formalin-fixed/paraffin-embedded specimens. Immunostainings against Ki-67 and Cox-2 antibodies were carried out by using polymer method as described in the kit’s instruction sheet. All data were dealt statistically with SPSS 10.0.Results Oral mucosa of 50 healthy people showed grossly ruddy and soft,but only 10 samples out of 50 healthy people showed histologically normal mucosa,other 40 samples showed epithelial hyperplasia and edema similar to the histopathology of leukoedema. Ten cases of leukoplakia showed epithelial hyperplasia and hyperorthokeratosis. Immunohistochemically,nuclear Ki-67 expressions were positively identified in 10 normal mucosa (5.24%),in 40 epithelial hyperplasia and edema (14.41%) and in 10 leukoplakia (12.08%). There was significantly different between the normal mucosa and both epithelial hyperplasia and leukoplakia (P<0.05),whereas no significant difference was noted between epithelial hyperplasia and leukoplakia (P>0.05). The expression of Cox-2 was gradually increased from 10 normal mucosa (0%) to 40 epithelial hyperplasia (55.0%) and 10 leukoplakia (70.0%),there was statistically different between the normal mucosa and both epithelial hyperplasia and leukoplakia (P<0.05),and no any significant difference was found between epithelial hyperplasia and leukoplakia (P>0.05).Conclusion Nearly 80% oral mucosa of healthy people in this study showed the histopathological changes.更多还原