【摘要】 背景:研究发现不同组织和细胞表达的4.1蛋白种类和剪切形式差别很大,不同4.1蛋白的不同结构域和细胞定位与其功能有必然的联系。目的:观察4.1蛋白家族中4.1R,4.1N,4.1G和4.1B在大鼠大脑皮质神经细胞中的mRNA和蛋白表达和细胞定位。方法:用RT-PCR,Western blot和免疫细胞化学染色及激光扫描共聚焦显微镜技术检测4.1蛋白家族在新生SD大鼠大脑皮质神经细胞中的mRNA和蛋白表达和在细胞中的定位。从细胞水平分析4.1蛋白家族中4.1R,4.1N,4.1G和4.1B的细胞内表达和定位的差异。结果与结论:4.1蛋白家族mRNA和蛋白在大鼠大脑皮质神经细胞均有所表达。4.1R主要表达在神经细胞胞膜和胞浆中,特别是突起延伸或轴突延伸部位高表达。4.1G主要表达在神经细胞的胞浆中,尤其是突起部位。4.1N主要表达在突起胞浆和胞膜。4.1B主要表达在胞体部位的胞浆和胞膜中。结果提示,4.1R,4.1G,4.1N和4.1B蛋白在体外培养的皮质神经细胞中均有不同程度的表达,并且在细胞内定位不同。更多还原

【Abstract】 BACKGROUND:Studies have showed that the protein 4.1 expression types and splicing vary significantly in different tissues and cells.The different structures of different protein domains and cellular localization of protein 4.1 are intrinsically linked.OBJECTIVE:To observe the expression of the mRNA,protein and the localization of 4.1R,4.1N,4.1G,and 4.1B in rat cortical neurons.METHODS:The expression of the mRNA,protein and the localization of protein 4.1 families in new born Sprague Dawley rats was detected by RT-PCR,Western blot,immunocytochemical staining and laser-scanning confocal microscopy.The differences of expression and localization of localization of 4.1R,4.1N,4.1G,and 4.1B in cells were compared.RESULTS AND CONCLUSION:Both mRNA and protein of 4.1R,4.1N,4.1G,and 4.1B in rat cortical neurons were expressed in vitro.4.1R mainly appeared in nerve cell membrane and cytoplasm,especially highly expressed in neurite extension or axon extension.4.1G mainly emerged in cytoplasm of nerve cells,especially the protruding parts.4.1N mainly located in cytoplasm and membrane processes.4.1B mainly expressed in cell bodies and in parts of the cytoplasm and membrane.The results demonstrated that the four kinds of protein 4.1 families in rat cortical neurons were expressed in vitro with distinct intracellular locations.更多还原