【摘要】 背景:白细胞介素15是一种重要的骨髓生长调节因子,可能具有刺激骨髓增生异常综征CD34+细胞增殖的作用。目的:观察白细胞介素15对儿童骨髓增生异常综合征造血细胞增殖的影响。方法:采用细胞计数、MTT抑制实验检测0,10,20,50,100,200μg/L白细胞介素15对18例1～14岁儿童骨髓增生异常综合征患者CD34+细胞增殖的影响,找出白细胞介素15作用的峰浓度。以白细胞介素15达到峰值的浓度为实验组,不加白细胞介素15的为对照组,培养12d以了解细胞最佳增殖时间。结果与结论:体外培养的骨髓CD34+细胞在培养第8天生长状况最好,并且随着白细胞介素15质量浓度的增加,其活细胞数也随着增加,当白细胞介素15为100μg/L时,其增殖状况最好;当白细胞介素15为200μg/L时,其增殖状况反而欠佳。在培养的第8天,白细胞介素15对骨髓增生异常综合征患者骨髓CD34+细胞抑制率最低,当其浓度在0～100μg/L范围内,随质量浓度增加,其抑制作用递减,而当其质量浓度>100μg/L其抑制作用有所增强。说明白细胞介素15呈时间和剂量依赖性地促进骨髓增生异常综合征患者骨髓CD34+造血干/祖细胞增殖。更多还原

【Abstract】 BACKGROUND:Interleukin(IL)-15 is a member of hematopoietic cytokine superfamily.It may stimulate the proliferation of the myelodysplastic syndrome(MDS) hematopoietic stem/progenitor cells.OBJECTIVE:To investigate the effects of IL-15 on proliferation of hematopoietic cells in MDS child.METHODS:Cell-counting,MTT-inhibitory test were used to examine the inhibitory effect of IL-15(0,10,20,50,100,200 μg/L) on the proliferation of MDS CD34+ cells in 18 children aged 1-14 years to find out the peak concentration of IL-15.IL-15 at peak concentration served as experimental group,whereas those without IL-15 as control group.12-day culture was done to understand optimal proliferation time of cells.RESULTS AND CONCLUSION:At day 8,CD34+cells cultured in vitro grew well.With increased IL-15 concentration,living cells number increased as well.When IL-15 was 100 μg/L,its proliferation status was the best,but when IL-15 was 200 μg/L,its proliferation status was below the mark.At day 8,the inhibitory rate of IL-15 on CD34+ cells was the lowest.When IL-15’s concentration was between 0 and 100 μg/L.With increased IL-15’s concentration,the inhibition decreased progressively.When IL-15 was above 100 μg/L,the inhibition reinforced on the contrary.These indicate that IL-15 can accelerate MDS bone marrow CD34+ hematopoietic stem/progenitor cell proliferation in a dose-and time-dependent manner.更多还原