【摘要】 背景:研究显示,以可降解生物陶瓷聚磷酸钙为基材,加入微量锶元素,通过调控材料结构如孔径、孔隙率等性能,可制备出有足够力学强度并可控降解的掺锶聚磷酸钙。目的:观察成骨细胞与掺锶聚磷酸钙的细胞相容性。方法:将兔成骨细胞与不同含锶质量分数(0～100%)的掺锶聚磷酸钙体外复合培养,进行形态学和功能测定。MTT法检测掺锶聚磷酸钙中成骨细胞的活力,并测定成骨细胞碱性磷酸酶活性。结果与结论:在倒置显微镜、扫描电子显微镜和激光共聚焦显微镜下观察到成骨细胞在掺锶聚磷酸钙的表面和孔隙内大量黏附、增殖和生长,并且分泌细胞外基质及细胞表面的大量微绒毛,显示成骨细胞在掺锶聚磷酸钙材料上生长形态良好;同时通过MTT和碱性磷酸酶测定表明细胞功能良好,其中含锶1%掺锶聚磷酸钙细胞增殖活性及碱性磷酸酶活性最高,对成骨细胞无毒性,具有良好的细胞相容性。更多还原

【Abstract】 BACKGROUND: It is reported that strontium-doped calcium polyphosphate with controllable degradability and enough mechanical strength could be synthesized by adding trace strontium into biodegradable calcium polyphosphate due to the modulation of material structure, such as pore size and porosity. OBJECTIVE: To study the cellular compatibility of strontium-doped calcium polyphosphate to osteoblasts. METHODS: The rabbit osteoblasts were co-cultured in vitro on the strontium-doped calcium polyphosphate, their morphology and biology were studied. MTT assay was applied to determine the osteoblast vitality in strontium-doped calcium polyphosphate and alkaline phosphatase activities were detected. RESULTS AND CONCLUSION: Under inverted microscope, scanning electron microscopy and confocal laser microscopy, the co-cultured osteoblast adhered, proliferated and grew normally on the surface and pores of strontium-doped calcium polyphosphate, and secreted extracellular matrix and massive microvillus on the cell surface. The osteoblast grew well on strontium-doped calcium polyphosphate; meanwhile, cellular function was shown to be well by MTT assay and alkaline phosphatase activities, 1% strontium-doped calcium polyphosphate achieve the maximum of cellular proliferation activity and alkaline phosphatase activities, no toxicity on osteoblasts and good cellular compatibility.更多还原