【摘要】 背景:研究表明降钙素具有抗骨吸收作用,对软骨有保护作用,但其具体机制尚不明确。目的:探讨降钙素是否通过Wnt/β信号通路对关节软骨细胞起保护作用。方法:分离培养雄性日本大耳白兔关节软骨细胞,随机分为对照组,白细胞介素1β组和降钙素组,后两组细胞于传代后的第2天加入白细胞介素1β(1×10-5g/L)模拟骨关节炎发生,降钙素组第4天换成含有降钙素的完全培养基。第6天采用免疫细胞化学法检测β连环蛋白的表达,以及实时荧光定量PCR法检测β连环蛋白与分泌型frizzled相关蛋白1mRNA的表达。结果与结论:降钙素组β连环蛋白和mRNA的表达显著低于白细胞介素1β组(P<0.05);白细胞介素1β组β连环蛋白和mRNA的表达显著高于对照组(P<0.05)。降钙素组分泌型frizzled相关蛋白1的mRNA表达显著高于白细胞介素1β组(P<0.05),白细胞介素1β组分泌型frizzled相关蛋白1mRNA表达显著低于对照组(P<0.05)。这些结果说明降钙素能抑制关节软骨细胞的进一步损伤,可能是通过Wnt/β连环蛋白信号通路对关节软骨细胞起保护作用。更多还原

【Abstract】 BACKGROUND: Researches have reported the protection function of calcitonin for chondrocytes via preventing bone resorption, but the mechanism remains unclear. OBJECTIVE: To assess whether calcitonin exerts a protective action through the Wnt signal pathway in chondrocytes. METHODS: The chondrocytes were separated from Japanese male rabbits and randomly divided into the control, interleukin 1 β and calcitonin groups. In the later two groups, interleukin 1 β (1×10-5 g/L) was added at 2 days after passage to simulate osteoarthritis, and then DMEM containing calcitonin was used to culture the cells in the calcitonin group at 4 days after passage. The expression of β-catenin protein was detected by immunocytochemistry, and the mRNA of β-catenin and secreted frizzled-related protein 1 (sFRP-1) were determined by real-time PCR. RESULTS AND CONCLUSION: The expression of β-catenin and its mRNA in the calcitonin group was obviously lower than that of the interleukin 1 β group (P<0.05). Compared with the control group, the expression of β-catenin and its mRNA in the interleukin 1 β group appeared to be greater (P<0.05). The expression of sFRP-1 mRNA was notably higher in the calcitonin group than that of the interleukin 1 β group (P<0.05), which was lower than the control group (P<0.05). The results demonstrated that calcitonin can inhibit the further damage of articular cartilage, which may play its protective role via Wnt/β-catenin signal pathway.更多还原