【摘要】 背景:为了验证透明质酸修饰后的壳聚糖材料能否作为神经支架材料,课题组在前期体外实验结果的延续下,进行体内试验观察。目的:比较经透明质酸、多聚赖氨酸修饰的壳聚糖支架及单纯壳聚糖支架在脑组织中的生物相容性及抑制瘢痕作用的优越性。方法:将3种支架植入Wistar大鼠脑皮质损伤区,术后3,7,14,28,56d分别取脑组织行苏木精-伊红染色、免疫组化观察,观察移植部位炎症反应及瘢痕组织形成、神经细胞生长情况。结果与结论:各组支架与脑组织相容性良好,用透明质酸修饰的壳聚糖复合支架组与其他壳聚糖支架组相比,支架周围炎症反应轻、支架周围GFAP阳性细胞数少,差异均具有显著性意义(P<0.01)。结果提示各组壳聚糖支架均具有良好的生物相容性,用透明质酸修饰的壳聚糖复合支架较其他壳聚糖支架抑制瘢痕作用明显,有利于神经细胞的生长,在组织工程应用中更具有优越性。更多还原

【Abstract】 BACKGROUND:In order to verify whether the hyaluronic acid-modified chitosan can serve as nerve scaffolds,the research group conducted in vivo test based on previous in vitro study.OBJECTIVE:To compare the biocompatibility and superiority of scar inhibition of hyaluronic acid-modified and poly-l-lysine modified chitosan scaffold,as well as pure chitosan scaffold in brain tissue.METHODS:Three kinds of scaffolds were implanted in the damage zone of rat cerebral cortex.At 3,7,14,28,56 days postoperation,brain tissues were harvested to undergo hematoxylin-eosin staining and immunohistochemistry.The inflammation,scar tissue formation and the growth of never cells in the implanted site were observed.RESULTS AND CONCLUSION:Each kind of scaffolds has a good biocompatibility with brain tissues.Hyaluronic acid modified chitosan scaffold showed lighter inflammation and less GFAP-positive cells around the scaffolds compared with other scaffolds.The differences were statistically significant(P<0.01).Results showed that chitosan scaffolds of each group have a good biocompatibility.The hyaluronic acid modified chitosan scaffold is superior to others to inhibit scar formation and helps to the growth of nerve cells,and shows its superiority in tissue engineering.更多还原