【摘要】 背景:由于良好的疗效和较低的不良反应,局部药物控制释放系统防止感染正在引起越来越多的关注。而静电纺丝制得的高分子纳米纤维,是一种良好的载药材料。目的:使用静电纺丝技术制备载不同含量盐酸四环素的聚乳酸-聚乙醇酸载药纳米纤维膜,着重观察其抑菌性能和细胞相容性。方法:以15～20kV的电压,0.3～0.5mL/h的流速使用静电纺丝技术制备载不同含量盐酸四环素的聚乳酸-聚乙醇酸载药纳米纤维膜。通过扫描电镜观察纳米纤维膜的形貌。检测载药率,绘制药物释放曲线,并用改良的Kirby-Bauer实验来观察载药纳米纤维膜的体外抑菌性能。用MG-63细胞来检测纳米纤维膜的生物相容性。结果与结论:载不同含量盐酸四环素的聚乳酸-聚乙醇酸载药纳米纤维直径均在360～470nm之间。且载药率都可以达到80%以上。载药量为10%的纳米纤维突释最大。载药纳米纤维膜可以有效的抑制金黄色葡萄球菌的生长但是对于MG-63细胞的黏附和增殖没有明显的不良影响。相比较而言,载药量为3%和5%的载盐酸四环素纳米纤维膜对于防止引导组织再生术后感染而言是较好的选择。更多还原

【Abstract】 BACKGROUND:Local drug controlled-delivery systems for anti-inflammatory are gaining increasing attention for bone defect regeneration because of their extended therapeutic effects and reduced adverse effects.Electrospun polymer nanofiber is a kind of good drug delivery vehicle.OBJECTIVE:To fabricate tetracycline hydrochloride-loaded polyglycolic acid(PLGA) nanofibrous membrane with varied tetracycline hydrochloride contents using the electrospinning technique,and to study their performance in preventing bacterial infections as well as their cell compatibility.METHODS:Tetracycline hydrochloride-loaded PLGA nanofibrous membranes were synthesized at 15-20 kV under a steady flow rate of 0.3-0.5 mL/h using an electrospinning technique.Through the images of scanning electron microscopy,we obtained morphological characters of the nanofibers.Drug entrapment efficiency was determined,drug release profiles were tested and in vitro antimicrobial activities were observed from modified Kirby-Bauer test.MG-63 cells were used to test the biocompatibility of nanofibrous membrances.RESULTS AND CONCLUSION:Average diameters of PLGA nanofibers with different tetracycline hydrochloride contents(0%,3%,5% or 10%) were all about 360-470 nm.Tetracycline hydrochloride entrapment efficiencies were well above 80% for all the PLGA membranes.The 10% drug loaded membranes exhibited the highest initial burst release.Tetracycline hydrochloride-loaded PLGA nanofibrous membranes were effective in inhibiting growth of staphylococcus aureus but had no adverse effect on the adhesion and proliferation of MG-63 cells.Comparatively speaking,electrospun PLGA/tetracycline hydrochloride membranes with drug loading of 3% and 5% are good choices for the prevention of bacterial infections in guided tissue regeneration.更多还原