【摘要】 背景:脂联素对胰岛素抵抗、代谢综合征有着重要的调节作用,其合成受过氧化物酶体增殖物活化受体γ活性的调节,研究表明部分血管紧张素受体拮抗剂可通过激活过氧化物酶体增殖物活化受体γ影响脂联素代谢,但具体机制尚不清楚。目的:探讨替米沙坦对脂肪细胞脂联素表达和分泌的影响,并与坎地沙坦进行比较。方法:体外培养、诱导分化3T3-L1脂肪细胞,分别用空白培养液、坎地沙坦(10μmol/L)和替米沙坦(10μmol/L)干预3T3-L1脂肪细胞,采用RT-PCR法、Western blot法和ELISA法检测脂肪细胞脂联素mRNA和蛋白表达及培养液中脂联素的分泌水平。结果与结论:与空白组和坎地沙坦组相比,替米沙坦组脂肪细胞脂联素的mRNA和蛋白表达水平明显增加(P<0.05或P<0.01),但各组间脂肪细胞培养液中脂联素的分泌水平差异无显著性意义(P>0.05)。说明替米沙坦可以明显增加脂肪细胞脂联素的表达,但并不增加脂联素的分泌水平,其促进脂联素表达的作用优于坎地沙坦。更多还原

【Abstract】 BACKGROUND:Adiponectin plays a regulatory role in insulin resistance and metabolic syndrome,and the synthesis of which is regulated by peroxisome proliferator-activated receptor γ(PPAR γ).Studies demonstrated that angiotensin receptor blocker can affect the metabolism of adiponectin via activating PPAR γ,but the precise mechanism remains unclear.OBJECTIVE:To investigate the effect of telmisartan on the adiponectin expression in 3T3-L1 adipocytes in vitro,and to compare the results with candesartan.METHODS:3T3-L1 adipocytes were cultured and differentiated in vitro.The differentiated 3T3-L1 adipocytes were cultured with vehicle or candesartan(10 μmol/L) or telmisartan(10 μmol/L).The expression level and secretary level of adiponectin were detected by RT-PCR,Western blot and ELISA.RESULTS AND CONCLUSION:Compared with the control and candisartan groups,telmisartan significantly increased the expression of adiponectin mRNA and protein in adipocytes(P < 0.05,P < 0.01),but there was no significant difference in secretion levels of adiponectin between groups(P > 0.05).The results demonstrated that telmisartan can increase the adiponectin expression in 3T3-L1 adipocytes,but can not increase the secretion by 3T3-L1 adipocytes.The promotive effect of telmisartan is superior to candesartan.更多还原