【摘要】 背景:近年来大量临床研究表明针刺风池、太冲、曲池等穴位能有效降低血压,可用于高血压,但对其治疗的分子机制尚未阐明。目的:观察针刺大鼠风池、太冲、曲池等穴位对丝裂原活化蛋白激酶信号转导调控系统的影响,从而探讨针刺治疗高血压的分子机制。方法:选取8月龄自发性高血压雄性Wistar大鼠14只,随机分为针刺组和模型组,每组7只;另选取同月龄正常血压雄性Wistar-Kyoto大鼠7只作为对照组。对针刺组大鼠采用电针针刺双侧风池、曲池和三阴交穴,毫针刺太溪和太冲穴。3周后采用RT-PCR方法检测各组大鼠主动脉组织丝裂原活化蛋白激酶磷酸酶1mRAN的表达,Western blot方法检测丝裂原活化蛋白激酶磷酸酶1、磷酸化细胞外信号调节激酶1/2蛋白表达。结果与结论:与对照组比较,模型组主动脉组织磷酸化细胞外信号调节激酶1/2蛋白表达水平升高,丝裂原活化蛋白激酶磷酸酶1mRNA及其蛋白表达水平降低(P<0.01);与模型组比较,针刺组主动脉组织磷酸化细胞外信号调节激酶1/2蛋白表达水平降低,丝裂原活化蛋白激酶磷酸酶1mRNA及其蛋白表达水平升高(P<0.05)。提示针刺治疗自发性高血压大鼠可能是通过调控丝裂原活化蛋白激酶信号转导途径,增强磷酸化细胞外信号调节激酶1/2蛋白表达,降低丝裂原活化蛋白激酶磷酸酶1蛋白表达,从而改善血管重塑,降低血压。更多还原

【Abstract】 BACKGROUND: In recent years, a large number of clinical studies have shown that acupuncture Fengchi (GB 20), Taichong (LR 3), Quchi (LI 11) points can effectively lower blood pressure, which can be used for the treatment of hypertension, but the molecular mechanism of this treatment has not been clarified. OBJECTIVE: To explore the molecular mechanism of acupuncture treatment of hypertension via observing the effects of acupuncture Fengchi (GB 20), Taichong (LR 3), Quchi (LI 11) points on the mitogen-activated protein kinase (MAPK) signal transduction system. METHODS: A total of 14 8-month male spontaneously hypertensive rats were randomly divided into the acupuncture and model groups, with 7 animals in each group. Additional 7 male Wistar-Kyoto rats were served as the control group. Rats in the acupuncture group received electroacupuncture at bilateral Fengchi (GB 20), Quchi (LI 11), and Sanyinjiao (SP 6) points, pin acupuncture at the Taixi (KI 3) and Taichong (LR 3) points. The expression of MAPK phosphatase-1 (MKP-1) mRNA, MKP-1 protein and phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK1/2) in aorta was detected by RT-PCR and Western blot at 3 weeks after operation. RESULTS AND CONCLUSION: Compared with the control group, the p-ERK1/2 level was increased and the MKP-1 mRNA and protein levels were decreased in the model group (P < 0.05). Compared with the model group, the p-ERK1/2 level was decreased and the MKP-1 mRNA and protein levels were increased in the acupuncture group (P < 0.05). The results revealed that acupuncture treatment of spontaneously hypertensive rats may be through regulation of MAPK signal transduction pathway to enhance p-ERK1/2 protein expression and reduce MKP-1 protein expression, thereby, improve vascular remodeling and decrease blood pressure.更多还原