【摘要】 背景:以往研究从病理切片、生物力学、生化检测等宏观角度证实中药具有促进骨折愈合的作用。目的:从分子生物学角度观察壮筋续骨汤对大鼠胫骨骨折愈合中核心结合子α1基因表达的影响。方法:选取3月龄雄性SD大鼠100只,建立闭合骨折髓内固定动物模型,造模后以抽签法随机分壮筋续骨汤中药组和对照组。壮筋续骨中药组在造模当天灌胃给予壮筋续骨汤,每次2mL,2次/d;对照组灌胃给予等量生理盐水,处死前1h各灌胃1d量。术后1,2,4,8,12周取骨折区组织,通过RT-PCR方法进行半定量分析核心结合子α1基因表达。结果与结论:两组核心结合子α1基因在骨折2周后开始表达并逐渐增强,且壮筋续骨汤组表达较对照组显著增强(P<0.05)。提示壮筋续骨汤能促进大鼠胫骨骨折愈合中的核心结合子α1基因表达增强。更多还原

【Abstract】 BACKGROUND: Previous research proved that Chinese herbs could promote bone fracture healing at levels of pathological section, biomechanics, and biochemical test. OBJECTIVE: To probe into the effect of Zhuangjin Xugu decoction recipe on the expression of core binding factor α1 (Cbfα1) during tibial fracture healing. METHODS: The model of closed fracture fixation was induced in marrow of 100 SD male rats aging 3 months, and the rats were then randomly divided into Zhuangjin Xugu decoction recipe group and control group. Rats in the Zhuangjin Xugu decoction group were continued to take Zhuangjin Xugu decoction, 2 mL per administration, twice a day. Rats in the control group were given the same volume of normal saline. Semi-quantitative analysis was used to detect the expression of Cbfα1 of bone tissues in regional fracture in the 1st, 2nd, 4th, 8th, and 12th weeks using RT-PCR method. RESULTS AND CONCLUSION: The Cbfα1 mRNA was detected at 2 weeks after bone fracture and gradually increased. The expression in the Zhuangjin Xugu decoction group was significantly higher than in the control group (P < 0.05). Zhuangjin Xugu decoction could increase the expression of Cbfα1 during tibial fracture healing.更多还原