【摘要】 背景:目前股骨头坏死模型还不能很好地模拟临床股骨头坏死情况,利用微波灭活提供了一种新的方法,但是其具体的标准尚未确定。目的:探讨微波灭活制作股骨头坏死模型的适宜温度和时间。方法:将48只大白兔按数字随机法分为4组,将微波天线插入兔股骨头,4组分别以不同的时间(10,20min)和温度(50,55,60℃)进行股骨头微波灭活,灭活后即刻、1,2,4,8和12周,观察兔行为、利用X射线、MRI检测股骨头影像,并观察股骨头切片的组织坏死及修复情况。结果与结论:50℃10min条件微波灭活1周后,部分兔股骨头骨髓组织凝固变性,8周时坏死骨小梁及骨髓组织完全吸收。55℃10min微波灭活1周后,兔股骨头骨髓组织凝固,2周时股骨头出现T1相信号减低、T2相信号增高区,4周时坏死与修复同时进行,12周时骨修复停止,骨坏死继续,股骨头开始塌陷。50℃20min和60℃10min微波灭活8周后,所有兔股骨头塌陷变形。由此认为,采用55℃10min微波灭活股骨头是制作兔股骨头坏死模型的适宜温度和时间,其股骨头变化符合临床病理变化过程。更多还原

【Abstract】 BACKGROUND:The existed femoral head necrosis (FHN) model can not reflex the clinical necrosis of femoral head correctly.Microwave heating provides a new approach for model preparation,but the concrete standard remains uncertain.OBJECTIVE:To study the optimal temperature and time in preparing a FHN model induced by microwave heating.METHODS:Totally 48 rabbits were randomly divided into 4 groups on the basis of the microwave temperature (50,55,60℃) and heating time (10,20 minutes).The microwave antenna was inserted into the rabbit femoral head.The rabbits were sacrificed immediately and at 1,2,4,8 and 12 weeks after operation.A series of examinations were performed including gross observation,X-ray,histology and MRI to observe the femoral head necrosis and repair status.RESULTS AND CONCLUSION:Marrow partially solidified in the group (50 ℃,10 minutes) at 1 week,and the osteonecrosis returned to normal at 8 weeks after operation.In the group (55 ℃,10 minutes),marrow was completely coagulated at 1 week and low signal on T1 weighted images and increased signal on T2 images were identified at 2 weeks.Osteonecrosis and repair occurred at the same time at 4 weeks.At 12 weeks,the osteonecrosis continued and the repair stopped,and the femoral heads started to collapse.All femoral heads collapsed at 8 weeks in group (50 ℃,20 minutes) and group (60 ℃,10 minutes).Accordingly,microwave heating is a good method in developing FHN model.55 ℃ and 10 minutes are the optimal temperature and time for the development of FHN model of rabbits induced by microwave heating.更多还原