【摘要】 背景:以往有很多学者通过发泡法将β-磷酸三钙与明胶复合得到引导组织再生材料,但将其采用静电纺丝技术制成纤维膜的报道很少。目的:制备新型引导组织再生膜,并比较其与聚乳酸,聚丙交酯/乙交酯膜的细胞毒性。方法:采用静电纺丝法制备β-磷酸三钙/明胶引导组织再生膜,通过扫描电子显微镜对纤维膜表面进行观察。通过MTT实验对电纺β-磷酸三钙/明胶膜与聚乳酸,聚丙交酯/乙交酯膜的细胞毒性进行对比。结果与结论:新型电纺β-磷酸三钙/明胶膜为多孔状,β-磷酸三钙颗粒呈结节状附着在明胶纤维表面,纤维直径平均为400～500nm,分布比较集中,大多在200～500nm之间。与聚乳酸,聚丙交酯/乙交酯膜及阴性对照之间细胞毒性差异无显著性意义(P>0.05)。说明新型电纺β-磷酸三钙/明胶引导组织再生膜细胞毒性较低,基本符合生物材料安全性的要求,有望成为新型的引导组织再生膜材料之一。更多还原

【Abstract】 BACKGROUND:In the past,guided tissue regeneration materials were produced by β-tricalcium phosphate(β-TCP)and gelatin(Gel),but the production of fiber membrane by electro-spinning technique was reported less.OBJECTIVE:To prepare a new kind of Gel with β-TCP hybrid nanofibrous membrane and testing its biocompatibility compared with polylactic acid(PLLA)and poly(lactide-co-glycolide)(PLGA)membrane.METHODS:The β-TCP/Gel guided tissue regeneration membrane was made by electro-spinning technique.Scanning electron microscopy(SEM)was employed to observe membrane surface.MTT test was performed to compare toxicity among β-TCP/Gel,PLLA,and PLGA membranes.RESULTS AND CONCLUSION:The β-TCP/Gel membrane was porous,and β-TCP granules were nodosity-adhered on surface of Gel.The average diameter of fiber was 500-600 nm.The distribution of fiber ranged from 200 to 500 nm.There was no significant difference in toxicity among PLLA,PLGA,and negative control group(P>0.05).The results suggested that the β-TCP /Gel membrane was low cytotoxicity and suitable for tissue engineering.It would be a promising material for periodontal tissue regeneration.更多还原