【摘要】 背景:研究证明,膝关节前交叉韧带切断后,基质金属蛋白酶13在关节软骨细胞外基质降解中起重要作用,然而基质金属蛋白酶13在外侧半月板退变中有何作用,作者尚未查及到文献报道。目的:探讨兔前交叉韧带切断后外侧半月板中基质金属蛋白酶13阳性表达率的变化和意义。方法:48只新西兰兔膝关节配对为实验侧和对照侧造模,造模后第1,3,6,8周各随机处死12只,通过大体观察和苏木精-伊红染色对外侧半月板退变进行评分,免疫组织化学检测基质金属蛋白酶13表达。结果与结论:随时间延长,与对照组比较,实验组外侧半月板退变评分逐渐增高(P<0.05)。各时间点实验组基质金属蛋白酶13阳性表达率均较对照组增高(P<0.05)。实验组基质金属蛋白酶13阳性表达率在3,6周显著高于1,8周(P<0.05),8周显著高于1周(P<0.05),3,6周差异无显著性意义(P>0.05)。结果提示基质金属蛋白酶13参与了外侧半月板组织的退变;基质金属蛋白酶13阳性表达率降低并不表示外侧半月板组织退变结束。更多还原

【Abstract】 BACKGROUND:Previous studies demonstrated that matrix metalloproteinase-13(MMP-13) plays a great role in anterior cruciate ligament(ACL) rupture-induced degradation of hyaline cartilage extracellular matrix of knee joint,however,the role of MMP-13 in the degeneration of lateral meniscus is poorly understood.OBJECTIVE:To investigate the changes and significance of the MMP-13 expression in the lateral meniscus of rabbit with ACL rupture.METHODS:Lateral meniscus of 48 rabbits was randomly divided into experimental side and control side,and 12 rabbits were randomly selected and sacrificed at 1,3,6 and 8 weeks after model preparation.The degeneration of lateral meniscus was scored through gross and haematoxylin-eosin staining,and the expression of MMP-13 was detected using immunohistochemistry.RESULTS AND CONCLUSION:With time prolonged,the scores of lateral meniscus degeneration were increased in the experimental group than that of the control group(P<0.05).Compared with the control group,the positive expression of MMP13 was greater in the experimental group at each time point(P<0.05).In the experimental group,positive expression of MMP13 was greater at 3 and 6 weeks than that of the 1 and 8 weeks(P<0.05),which was significantly higher at 8 weeks than 1 week(P<0.05),however,there was no significant difference between 3 and 6 weeks(P>0.05).MMP-13 maybe a promotional factor which causes the degeneration of lateral meniscus;the decreasing of the MMP-13 expression is not a stopping signs of lateral meniscus degeneration.更多还原