【摘要】 目的:研究激发型CD40单抗(5C11)对宫颈癌细胞株SiHa化疗敏感性的影响及其机制。方法:采用流式细胞术检测宫颈癌细胞株SiHa上CD40分子的表达,用MTT法检测5C11联合化疗药物对SiHa细胞的作用,PI染色检测SiHa细胞周期的变化,Annexin V-PI法检测细胞凋亡,RT-PCR方法分析单抗5C11作用SiHa细胞后凋亡基因表达水平变化。结果:宫颈癌细胞株SiHa高表达CD40,5C11和盐酸吉西他滨单独抑制细胞生长作用不明显,但两者联合能显著抑制SiHa生长和促进凋亡,SiHa细胞经5C11作用后出现S期阻滞,抗凋亡基因bcl-XL表达明显下调,促凋亡基因Bax的上调作用不明显。结论:CD40分子通过介导S期阻滞和调节凋亡基因表达水平增加SiHa细胞对盐酸吉西他滨化疗的敏感性。更多还原

【Abstract】 Objective:To study the enhancing effect of CD40 mAb on the chemosensitivity of human cervical tumor cell line SiHa to Gemcitabin.Methods:Flow cytometric analysis was used to detect the expression of CD40 in SiHa.Agonistic anti-human CD40 monoclonal antibody(5C11) was added to the cell culture system.PI staining and Annexin V-PI assay were used to study the biological effect of 5C11 on cervical tumor cell.Cell proliferation was analyzed by MTT assay.Expression of apoptotic genes was evaluated by RT-PCR.Results:The cervical tumor cell line SiHa highly expressed CD40.5C11 or Gemcitabin could not effectively induce SiHa proliferation arrest,but combination of 5C11 and Gemcitabin could do.CD40 activation induced arresting the cells at S phases and down-regulating anti-apoptotic gene bcl-XL expression and up-regulating apoptotic gene Bax expression weakly.Conclusion:CD40 activation can enhance chemosensitivity of human cervical tumor cell to Gemcitabin by inducing arresting the cells at S phases and affecting apoptotic genes expression.更多还原