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清创术中生理盐水冲洗创面的合适用量的动物实验研究
Study on Animal Experiments about Appropriate Amount of Physiological Saline Flushing Injured Area in epluchage
【摘要】 目的:通过对兔四肢骨及肌肉横断面的有条件污染后,用不同量生理盐水冲洗,分别测定冲洗后断面细菌残留菌落数,进行比较,探讨清创术中生理盐水冲洗创面的合适用量(或不得少于的量)。方法:使用体重>2.5kg的家兔,在肩关节和髋关节处截断,暴露骨和软组织的断面,测量断面面积后,用泥沙加压20kg污染之,在自然环境中停放4小时后,按1cm×1cm创面用生理盐水10ml~60ml依次均分为6组,在相同条件下冲洗后,分别取样,做细菌培养。结果:经统计学处理、分析,使用6个用量组生理盐水冲洗创面后,第5组的创面残留细菌菌落数与第1、2、3、4组均有明显差异(p<0.01),与第6组无明显差异。结论:在本实验条件下的清创术中,使用生理盐水冲洗创面的合适用量为50ml/cm2(或不得少于50ml/cm2)。
【Abstract】 Objective:Flushing with uncommensurability physiological salines the rabbit’s arms and leg’bone and the muscle cross secticns which were polluted under given conditions,we could discuss the appropriate amount(or should not be less than the amount)of the physioloycal saline flushing the injured area in the epluchage.Methods:After cutting off the bodyweight> 2.5kg domestic rabbit’s shculder joint and hip joiont esposing the bone and soft tissue cross-section and polluting them with 20kg sitt pressuring after measuring the area,we could park them in the natural envircnment for 4hours and then divide equally them 6grcups in turn according to each square centimeter injured area with 10ml-60ml physiological saline.Then we take samples to make the bacilliculture separately.Results:Through statistics handling,analysing,the 5th grcup of injured area residual bacterial clump number with the lst.2、3、4grcups had the obvicus difference(p<0.01),with the 6th group of not obvicus difference.Conclusion:The appropriate amount using the physiological saline to flush the injured area should 50ml/cm2(or did not be short in 50ml/cm2)under this experimental condition’s epluchage.
- 【文献出处】 中国伤残医学 ,Chinese Journal of Trauma and Disability Medicine , 编辑部邮箱 ,2010年01期
- 【分类号】R641
- 【被引频次】6
- 【下载频次】85