【摘要】 利用免疫荧光-激光共聚焦显微技术及透射电镜技术观察研究了嘉庚蛸精子形成过程中微管的分布特征及其作用。结果表明,精细胞早期,核呈圆形或卵圆形,核内染色质由团块状趋向颗粒状均布、或部分凝集成絮状,核周无微管套结构、且微管不均匀分布;精细胞中期,核呈橄榄形,核内染色质呈颗粒状或絮状,微管在核周均匀分布、形成微管套;精细胞后期,核呈长梭形,核内染色质呈纤维状,微管套紧贴核周;未成熟精子,核呈长柱状,核内染色质致密均布,微管套仍紧密围绕核周;成熟精子,核周微管结构消失。嘉庚蛸精子形成过程中微管结构有一个动态变化过程,我们认为,微管套可能在核的形态发生过程中发挥重要作用;由于微管套只在精细胞核形变过程中存在和起作用,因此,微管套可能通过特殊的途径参与核内染色质的浓缩。更多还原

【Abstract】 Immunofluorescence-based laser confocol microscopy and transmission electron microscopy were employed to study the dynamic distribution and potential function of microtubule during spermiogenesis in Octopus tankahkeei.At early spermatid stage,a round or oval nucleus was observed,chromatin changed from conglomeration to granule,and then it became flocculent.No microtubular structure-manchette was found,and the microtubules exhibited an uneven perinuclear distribution.At middle spermatid stage,the nucleus was olive-like in shape,the chromatin became to condense and granular or flocculent structure could be found in the nucleus, manchette was formed which surrounded the nucleus.At late spermatid stage,the nucleus showed a long shuttlelike shape,the chromatin became fibrillar,and manchette were tightly attached to the nucleus periphery.Before the mature sperm was formed,the nucleus was a long cylindrical in shape,the chromatin was highly condensed and homogeneously distributed,and the manchette still closely associated with the nucleus.In mature sperm,no manchette was found.We propose that the nuclear morphogenesis during Octopus tankahkeei spermiogenesis is conducted by the special microtubular structure-manchette;because the manchette only appear during spermiogenesis,it may have functions during chromatin condensation.更多还原