【摘要】 以中华猕猴桃伏牛95-2（Actinidia chinensis‘Funiu 95-2’）叶片为试验材料,对不同侵染方式、预培养时间、菌液浓度、共培养时间等影响β-葡萄糖苷酸酶基因（β-glucuronidase,GUS）瞬时表达率的因素进行了研究。结果表明,叶片预培养3天,菌液浓度A₆₀₀值为0.3,真空渗入方式侵染10 min,共培养4天条件下,GUS基因瞬时表达率最高,达到92.2%。对转基因抗性植株进行PCR检测和GUS组织化学染色,初步证明外源基因已整合到中华猕猴桃伏牛95-2的基因组中。更多还原

【Abstract】 Factors affecting transient expression frequency of GUS gene,including different infection methods,days of pre-culture,concentration of Agwbacterium tumefaciens,days of co-culture were studied in genetic transformation,using leaves of Acitnidia chinensis‘Funiu 95-2’ as explants.The results showed that the highest transient expression frequency（92.2%） was obtained when explants were firstly pre-cultured for 3 d, infected for 10 min with Agwbacterium tumefaciens(A₆₀₀=0.3) by vacuum infiltration and finally co-cultured for 4 d.PCR detection and GUS histochemical staining proved initially that the foreign gene had been integrated into the Acitnidia chinensis genomes.更多还原