【摘要】 目的:利用大鼠肠易激综合征(irritable bowel syndrome,IBS)模型,观察电针对IBS大鼠肠道运动功能异常的作用,为临床治疗IBS提供科学依据。方法:采用Sprague-Dawley新生雄性幼鼠制作IBS大鼠模型,观察模型大鼠结肠蠕动的异常变化,探讨电针对IBS大鼠肠道运动功能异常的治疗效应。实验分为4组(n=6):正常组、模型组、假电针组和电针组;穴位采用双侧"足三里"和"上巨虚";电针采用100Hz/2Hz疏密波,刺激强度为1、2、3mA(每隔10min增加一次),单次治疗30min。结果:与正常组相比,成年IBS大鼠结肠蠕动频率明显增加(P<0.05);电针治疗后30min内IBS大鼠结肠蠕动频率明显下降(P<0.05);而假电针组IBS大鼠结肠蠕动频率没有明显变化(P>0.05)。结论:IBS大鼠的肠道运动有异常增加,电针对IBS大鼠异常增加的肠道运动有明显的抑制作用。更多还原

【Abstract】 Objective:To investigate the effects of electroacupuncture (EA) on intestinal motility disorder in rats with irritable bowel syndrome (IBS) in order to provide experimental evidence for improving clinical treatment to IBS with EA.Methods:IBS was induced by inserting a balloon into the rectum of male Sprague-Dawley rats from their neonatal age (8th,21st day after birth) and applying colorectal distention stimulation by inflating air into the balloon. Peristaltic wave (PW) was used to estimate intestinal motility. Rats were divided into 4 groups (n=6):normal group,untreated group,sham EA group and EA group. Acupoints of Zusanli (ST36) and Shangjuxu (ST37) in the hind limbs bilaterally were chosen for EA and sham EA treatment. Trains of dense-sparse frequencies (100 Hz and 2 Hz alternately) and intensity of 1,2 and 3 mA (10 min for each) were applied for 30 min with each EA treatment while inserting similar needles without electrical stimulation was done as sham EA treatment. PW recorded for 30 min in normal and untreated groups respectively was taken as control values,while PW recorded after EA or sham EA treatment for 30 min was taken as responsive values. Results:Frequency of PW was significantly increased in the untreated group as compared with that in the normal group (P<0.05),and decreased after 30-min EA treatment (P<0.05). There was no significant change in the PW of sham EA group as compared with the control group and before treatment (P>0.05). Conclusion:These results suggest that 1) there is an abnormal increase in intestinal motility indicating an intestinal motility disorder in IBS rats; 2) EA but not sham EA can relieve such intestinal motility disorder.更多还原