【摘要】 采用形态学、拮抗试验、酯酶同工酶及RAPD技术对7种桑黄菌株进行了种性分析,并比较了4种方法分析结果的异同,虽稍有差异,但总体分类一致.由于单一方法存在局限性,且不同菌丝生长阶段对实验结果影响较大,所以还需对其亲缘性从DNA分子水平进行考察.根据RAPD扩增结果,构建反映了菌株间亲缘关系的聚类图,可将7个菌株划分为4大类,直观准确地揭示菌株间的差异性并加以鉴别.更多还原

【Abstract】 In this study,morphology,antagonistic test,esterase isozyme and RAPD technology ware used to analyse the classification of 7 Phellinus igniarius cultivars,and compared the results of the similarities and differences.Although slightly different,the overall classification was agreement.As the limitations of a single method,and the different stages of mycelial has a greater impact on the experimental results,it required from the molecular level to investigate the affinity between them.A dendrogram showed that genetic relationships was constructed through an unweighted pair-group method(UPGMA) based on the DNA polymorphism and the 7 cultivars were clustered into 4 main groups,which was helpful to find out the difference among the test strains and to distinguish them directly.更多还原