【Objective】 The effects of several metal ions on the growth of Streptomyces gilvosporeus and biosynthesis of natamycin were investigated.【Method】 Shaking flask fermentation with single factor comparison experiment was adopted.【Result】 Results showed that Mg2+ and Ca2+ could promote the growth of S.gilvosporeus,Fe2+,Cu2+,Mn2+ and Co2+ inhibited the growth of S.gilvosporeus,while Na+,K+ and Zn2+ had no obvious effects on the growth of S.gilvosporeus.Adding appropriate concentrations of K+,Ca2+and Co2+ into pr...