【摘要】 目的:观察重组Hap蛋白免疫抗不可分型流感嗜血杆菌(NHTi)感染的免疫保护效果,初步探讨其保护机制。方法:用纯化的Hap重组蛋白与黏膜免疫佐剂霍乱肠毒素B亚单位(CT-B)联合鼻腔免疫C57BL/6小鼠。NHTi琼脂珠悬液对免疫小鼠进行气管内注射,建立小鼠NHTi急性肺部感染模型。NHTi攻击5d后,收集实验小鼠的支气管肺泡灌洗液(BALF)进行白细胞计数,并取其肺组织进行肺部荷菌数检测及肺组织的病理检测。结果:Hap重组蛋白与CT-B的联合免疫能显著降低小鼠BALF中的白细胞总数、中性粒细胞渗出(P<0.05)及感染小鼠肺组织内NTHi的荷菌数,并减轻感染小鼠支气管、细支气管周围及肺泡内的炎性浸润程度,明显改善其肺组织的病变。结论:鼻腔免疫的Hap重组蛋白在动物体内能发挥良好抗NTHi感染的免疫保护作用,为NTHi新型疫苗的研发提供了理论基础和实验依据。更多还原

【Abstract】 AIM:To observe the immune effect of Hap recombinant protein on murine model of bronchopneumonia infected with NTHi,and explore the mechanism about the anti-NTHi infection.METHODS:The C57BL/6 mice intranasally immunized with purified Hap recombinant protein and CT-B were challenged by NTHi encased in agar beads.The immunifaction of anti-infection was observed through encocyte counting of BALF,bacteria detection of lung and the pathologyical change of lung tissue.RESULTS:In the challenge with NTHi experiment,the inflammatory exudation of the infected murine and pathological change of lung tissue was relieved by combined immunization of Hap recombinant protein and CT-B,and quantity of NTHi in lung of the infected murine was reduced obviously.CONCLUSION:The Hap recombinant protein also had good ability of anti-NTHi infection in the murine model of NTHi bronchopneumonia.This study could offer the oretical and experimental basis for development of new vaccine against NTHi.更多还原