【摘要】 目的探讨临床I期(cI期)非小细胞肺癌(NSCLC)中血管内皮生长因子C(VEGF-C)的表达、微淋巴管密度(MLVD)、淋巴结转移及其与化疗的关系。方法应用免疫组化SP法检测cI期NSCLC癌组织、癌旁组织及正常肺组织中VEGF-C的表达,计数MLVD,检测血清VEGF-C蛋白含量,并结合病理淋巴结转移情况和化疗前、后VEGF-C蛋白含量的变化进行统计学分析。结果在NSCLC中,VEGF-C呈过度表达,与癌旁组织和正常肺组织相比差异有统计学意义(P<0.01);在癌组织中淋巴结阳性组和淋巴结阴性组的VEGF-C阳性表达率(91.9%vs.76.7%)、MLVD[(20.0±4.8)vs.(10.6±2.2)]差异有统计学意义(P<0.01);血清VEGF-C蛋白含量与组织检测VEGF-C具有较好的相关性(r=0.848,P<0.01),化疗患者化疗前血清VEGF-C蛋白含量(237±72.3)ng/mL较化疗后(178±69.2)ng/mL变化明显(P<0.01)。结论 VEGF-C在cI期NSCLC中过度表达与淋巴结转移密切相关;血清VEGF-C蛋白含量测定是一种有效的VEGF-C检测方法;化疗前、后血清VEGF-C蛋白含量的变化可以预测化疗效果。更多还原

【Abstract】 Objective To study the correlation of vascular endothelial growth factor-C(VEGF-C) expression and lymphatic microvessel density(MLVD) with lymphatic metastasis and sensitivity of chemotherapy in non-small cell lung cancer(NSCLC) of clinical stage Ⅰ.Methods VEGF-C expression and MLVD were detected in cancer tissues,para-cancer tissues of clinical stage I NSCLC and normal lung tissues.The clinico-pathological results of lymphatic metastasis and VEGF-C protein changes after chemotherapy were analyzed statistically.Results In cancer tissues of NSCLC,the over-expression of VEGF-C was significantly higher than that in para-cancer tissues and normal lung tissues(P<0.01).The positive rates of VEGF-C expression(91.9% vs.76.7%) and MLVD [(20.0±4.8) vs.(10.6±2.2)] in lymphatic metastasis group were significantly higher than those in groups without lymphatic metastasis(P<0.01).The detection of VEGF-C in serum had a close correlation with VEGF-C protein in tissues(r=0.848,P<0.01).The level of VEGF-C protein in serum before chemotherapy(237±72.3)ng/mL was significantly reduced after chemotherapy [(178±69.2)ng/mL,P<0.01)].Conclusion Over-expression of VEGF-C is closely related to lymphatic metastasis in NSCLC of clinical stage Ⅰ.The detection of VEGF-C in serum is helpful in clinical stage;changes of VEGF-C protein in serum before and after chemotherapy can predict the results of chemotherapy.更多还原