【摘要】 目的探讨肠神经系统在大鼠重症急性胰腺炎肠动力紊乱时的变化以及柴芍承气汤对大鼠肠道肌间神经丛内一氧化氮合成酶(nNOS)神经元表达的影响。方法将30只SD大鼠随机分为3组:假手术组、模型组、柴芍承气汤组(CSCQD组);模型组和CSCQD组经胰胆管逆行注射质量浓度为50 g/L的牛磺胆酸钠建立大鼠SAP模型后,分别给予生理盐水和CSCQD药液。测定大鼠的胃肠传递时间,HE染色光镜下观察胰腺的病理变化,用双重免疫荧光染色法观察回肠及结肠肌间神经丛NOS神经元的变化。结果与模型组相比,CSCQD组传递指数(TI)明显升高(P<0.05),胰腺组织病理损害明显减轻(P<0.05),NOS神经元占总神经元的百分比明显降低(P<0.05)。结论CSCQD通过逆转肠神经系统NOS神经元的可塑性变化,改善SAP大鼠的肠动力,同时减轻了胰腺的病理损伤。更多还原

【Abstract】 Objective To investigate the changes of enteric nervous system in gastrointestinal motility disorder in rats with severe acute pancreatitis(SAP) as well as the influence of Chaishao Chengqi Decoction on neuronal nitric oxide synthase neuron(nNOS) neuron expression in myenteric plexus of rat intestinal tract.Methods SD rats were randomly divided into 3 groups(10 for each): sham operation group,model group and Chaishao Chengqi Decoction(CSCQD) group.SAP model was established in model group and CSCQD group rats by retrograde injection of 50g/L sodium taurocholate into the junction of the common bile duct and pancreatic duct.Model group was administered with normal saline and CSCQD group was administered with Chaishao Chengqi Decoction.The gastrointestinal transmission time was observed.Pathological changes of the pancreas were examined with HE staining under light microscope.The neuronal nitric oxide synthase neurons in the small intestines and colonic myenteric plexus were detected by the dual immunofluorescent staining.Results Compared with that in model group,transmission index was increased in CSCQD group(P<0.05).The pathological lesion of the pancreas in CSCQD group was significantly lessened(P<0.05).The percentage of NOS-immunoreactive neurons was significantly decreased(P<0.05).Conclusion By reversing the plasticity of NOS-immunoreactive neurons,Chaishao Chengqi Decoction improves the gastrointestinal motility in rats with SAP and reduces the pathological damages of the pancreas.更多还原