【摘要】 目的:探讨硼替佐米对核因子-κB(NF-κB)活化的干预作用及对人LOVO细胞增殖的影响。方法:体外培养人LOVO结肠癌细胞并给予不同浓度的硼替佐米,以Western blot和ELISA法分别检测IκBα和NF-κB的表达;以MTT法和Annexin V-FITC/PI双色标记法分别检测细胞增殖和细胞凋亡的变化。结果:硼替佐米作用LOVO细胞后,胞浆IκBα和NF-κB的水平逐渐上升,而胞核NF-κB的水平明显降低(P<0.01),并呈一定的浓度依赖性,高浓度时作用最明显(P<0.01);硼替佐米能明显抑制LOVO细胞的增殖,并呈一定的剂量和时间依赖性。随着硼替佐米浓度的上升,细胞凋亡率明显增加(P<0.01)。结论:硼替佐米通过抑制IκBα的降解能有效地干预NF-κB活化,抑制LOVO细胞的增殖并诱导细胞发生凋亡。更多还原

【Abstract】 Objective: To investigate the influences of LOVO cells of the intervention of nuclear factor-κB (NF-κB) activation by bortezomib on the proliferation.Methods: LOVO cells was cultured in vitro and treated with different concentrations of bortezomib.The expressions of I κ B α and NF-κB were analyzed by western blot and enzyme linked immunosorbent assay,respectively.The changes of cell growth and apoptosis were investigated by MTT assay and Annexin-V/PI double dyeing assay,respectively.Results: In bortezomib-treated cells,the expressions of IκBα and NF-κB in cytopalsm were obviously upregulated,while the level of NF-κB in nucleus was sharply downregulated in a dose-dependent manner (P <0.01),which was the most effective at the concentration of 80 nmol/L (P <0.01).Bortezomib significantly inhibited the proliferation of LOVO cell in a doseand timedependent manner.The percentage of apoptotic cells in bortezomib group was higher than that in control one (P <0.01).Conclusion: Bortezomib can effectively intervene NF-κB activation in LOVO cells by suppressing the degradation of I κ B α,inhibit the proliferation of LOVO cells and induce cell apoptosis.更多还原