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一株北极小球藻Chlorella sp.的分离鉴定及其对增强的UV-B辐射的生理生化响应
The Isolation and Identification of an Arctic Microalgae and Its Physiological and Biochemical Responses to Enhanced UV-B Radiation
【摘要】 为了了解微藻对UV-B辐射增强效应的响应,以一种分离纯化于北极冰川融水的淡水微藻(Chlorella sp.)为实验材料,在不同强度UV-B辐射下对其生长、生化组分和细胞超微结构等进行了研究。研究结果显示:3种不同强度的UV-B(22μW/cm2,45μW/cm2,70μW/cm2)辐射均可导致藻的比生长速率及色素含量下降,且辐射强度越强,两者的下降越明显;而MDA含量和SOD活性会随辐照强度的增强而提高。表明辐射强度增强,UV-B对藻的伤害程度加大,而该小球藻SOD活性随UV-B强度增强而提高,表明其对上升的UV-B辐射有一定的适应能力。
【Abstract】 The response of a microalgae(Chlorella sp.)strain which isolation and purification from Arctic glacial meltwater to the enhancement of UV-B radiation was explored in this study.The effects of three different UV-B radiation,i.e.,22 μW/cm2,45 μW/cm2,70 μW/cm2 were tested on the algal growth,biochemical components,and cell ultrastructure.The results showed that UV-B irradiation could inhibit the relative growth and reduce content of pigment;and the inhibiting effects were more significant according to the intensity increasing.Meanwhile,the SOD activity and MDA contents of the strain increased with higher UV-B irradiation.The results showed that the enhancement of UV irradiation could deepen the damage of Chlorella species and algae could adapt the higher intensity of UV-B radiation to some extent.
【Key words】 UV-B radiation; Photosynthesis pigments; Relative growth; SOD; MDA;
- 【文献出处】 武汉植物学研究 ,Journal of Wuhan Botanical Research , 编辑部邮箱 ,2010年01期
- 【分类号】Q945
- 【被引频次】4
- 【下载频次】200