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一株北极小球藻Chlorella sp.的分离鉴定及其对增强的UV-B辐射的生理生化响应

The Isolation and Identification of an Arctic Microalgae and Its Physiological and Biochemical Responses to Enhanced UV-B Radiation

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【作者】 彭小伟柳俊李运广罗玮蔡明红何剑锋

【Author】 PENG Xiao-Wei1,2,LIU Jun1,LI Yun-Guang1,LUO Wei2,CAI Ming-Hong2,HE Jian-Feng2(1.Life Science and Technology College,Huazhong Agricultural University,Wuhan 430070,China;2.Key Laboratory for Polar Science of the State Oceanic Administration,Polar Research Institute of China,Shanghai 200136,China)

【机构】 华中农业大学生命科学技术学院中国极地研究中心国家海洋局极地科学重点实验室

【摘要】 为了了解微藻对UV-B辐射增强效应的响应,以一种分离纯化于北极冰川融水的淡水微藻(Chlorella sp.)为实验材料,在不同强度UV-B辐射下对其生长、生化组分和细胞超微结构等进行了研究。研究结果显示:3种不同强度的UV-B(22μW/cm2,45μW/cm2,70μW/cm2)辐射均可导致藻的比生长速率及色素含量下降,且辐射强度越强,两者的下降越明显;而MDA含量和SOD活性会随辐照强度的增强而提高。表明辐射强度增强,UV-B对藻的伤害程度加大,而该小球藻SOD活性随UV-B强度增强而提高,表明其对上升的UV-B辐射有一定的适应能力。

【Abstract】 The response of a microalgae(Chlorella sp.)strain which isolation and purification from Arctic glacial meltwater to the enhancement of UV-B radiation was explored in this study.The effects of three different UV-B radiation,i.e.,22 μW/cm2,45 μW/cm2,70 μW/cm2 were tested on the algal growth,biochemical components,and cell ultrastructure.The results showed that UV-B irradiation could inhibit the relative growth and reduce content of pigment;and the inhibiting effects were more significant according to the intensity increasing.Meanwhile,the SOD activity and MDA contents of the strain increased with higher UV-B irradiation.The results showed that the enhancement of UV irradiation could deepen the damage of Chlorella species and algae could adapt the higher intensity of UV-B radiation to some extent.

【关键词】 UV-B辐射光合色素比生长率SODMDA
【Key words】 UV-B radiationPhotosynthesis pigmentsRelative growthSODMDA
【基金】 国家自然科学基金(40806073,40576002);科技部资源平台项目(2005DKA21406);上海市科委国际合作项目(052307053);国家海洋局青年基金(2008128);中国极地研究中心极地科学青年创新基金(JDQ200701)
  • 【文献出处】 武汉植物学研究 ,Journal of Wuhan Botanical Research , 编辑部邮箱 ,2010年01期
  • 【分类号】Q945
  • 【被引频次】4
  • 【下载频次】200
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