【摘要】 目的分析西安地区耐乙胺丁醇(EMB)结核杆菌临床分离株embB基因突变的特点,建立快速检测结核分枝杆菌乙胺丁醇耐药性的方法。方法应用聚合酶链反应-单链构象多态性(PCR-SSCP)和聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测104株结核分枝杆菌临床分离株的embB基因。结果以H37Rv标准株为对照,35株药物敏感株的SSCP和RFLP分析结果均与标准株一致,不存在突变;69株耐乙胺丁醇分离株中,39株(56.52%)SSCP泳动与标准株不一致,存在基因异常;19株(27.54%)RFLP分析存在基因异常。结论西安地区结核杆菌临床分离株对乙胺丁醇耐药与embB基因(尤其是306位密码子)突变有关,PCR-SSCP和PCR-RFLP技术可快速、准确地检测结核分枝杆菌对EMB的耐药性。更多还原

【Abstract】 Objective To analyze the characteristics of embB gene mutation in ethambutol-resistant Mycobacterium tuberculosis (M.TB) strains isolated from patients in Xi’an for establishing a rapid method to detect the mutation of embB gene in M.TB.Methods The embB gene of 104 Mycobacterium tuberculosis isolates was detected by PCR-Single Strand Conformation Polymorphism ( PCR-SSCP) and PCR-Restriction Fragment Length Polymorphism(PCR-RFLP).Results Taking strain H37Rv as a control,the SSCP and RFLP profiles of embB of 35 drug-sensitive strains in 104 Mycobacterium tuberculosis isolates were normal.The display of SSCP profiles in 39 of 69 (56.52% ) ethambutol-resistant isolates and the display of RFLP profiles in 19 of 69 (27.54% ) ethambutolresistant isolates were abnormal.Conclusion The EMB-resistance of M.TB strains isolated from patients in Xi’an is related to the mutation of embB gene,PCR-SSCP and PCR-RFLP are sensitive methods for detecting ethambutolresistance of M.tuberculosis.更多还原