【摘要】 目的探讨氟对大鼠生精细胞Fas蛋白表达影响。方法将30只4～5周龄雄性Wistar大鼠随机分为对照组、低剂量组、高剂量组。分别按0、10和20mg/kg染毒剂量隔日灌胃氟化钠(NaF)染毒39天,采用酸浸-氟离子选择电极法测定睾丸氟含量,用放射免疫法测定大鼠血清睾酮含量,用免疫组化(ICH)检测睾丸中Fas蛋白表达情况,同时镜检成熟精子质量。结果各染氟组大鼠睾丸氟含量均高于对照组(P<0.05)且随染毒剂量的增加而逐渐升高;各染氟组大鼠血清睾酮含量低于对照组(P<0.05),且随着染毒剂量的增加,睾酮含量逐渐降低;各染氟组大鼠生精细胞Fas蛋白阳性表达率高于对照组(P<0.05),且随着染毒剂量的增加,Fas蛋白阳性表达率逐渐升高;各染毒组大鼠精子数量和精子活动率低于对照组(P<0.05);精子畸形率高于对照组(P<0.05)。结论染氟雄性大鼠血清睾酮水平降低,激活了生精细胞Fas/FasL系统,从而导致生精细胞损伤。更多还原

【Abstract】 Objective To research the effect of fluorine on the expression of Fas protein,then study the mechanism of male reproductive toxicity induced by fluoride on molecular level.Methods Thirty Wistar male rats were divided into control group,low-dose group and high-dose group.The NaF dosage for every group were 0,2 and 4g /L.The content of NaF in testis was measured by using fluorine selective electrode.Changes of testosterone and Fas protein were observed using the methods of radioimmunoassay,in situ hybridization.In addition,we observed the quality of spermatozoa.Results The testis fluoride content of two fluorine treatment groups were higher than that of control group(P < 0.05),and had a dose-dependent effect.The level of testosterone,the number and the livability of the spermatozoon in fluorotic groups were lower than those of control rats(P < 0.05),and the above indexes decreased with the incrase of dosage.The expression of Fas in spermatogenic cells and the sperm aberration of each fluorotic group were higher than control group(P < 0.05),both of them increased with the increase of dosage.Conclusion Fluorin could reduce the level of serum testosterone,then activated the Fas /FasL system,which caused damage to the reprodutive system.更多还原