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喜树内生放线菌多样性及抗菌活性评价
Diversity and Antimicrobial Activities of Endophytic Actinomycetes Isolated from Camptotheca acuminata Decne.
【摘要】 从采集于云南大学的喜树中分离到了90株内生放线菌,经16SrRNA基因序列分析鉴定,分布于6个科10个属。对所有分离到的菌株进行抗菌活性检测,发现33.4%的菌株有抗菌活性。通过PCR方法,检测了PKSⅠ、PKSⅡ和NRPS基因,阳性检出率分别为31.1%,48.9%和45.6%。
【Abstract】 Ninety endophytic actinomycetes were isolated from surface-sterilized samples of Camptotheca acuminata Decne.collected from Yunnan University.Results of 16S rRNA gene sequences analysis showed that they belong to 10 genera,6 families.Among them,33.4% of endophytic actinomycete cultures presented antimicrobial activity.The polyketide synthasesⅠ(PKS-I),polyketide synthases Ⅱ(PKS-II) and non-ribosomal peptide synthase(NRPS) sequences were respectively detected by PCR in 31.1%,48.9% and 45.6% of tested strains.
【关键词】 喜树;
内生放线菌;
多样性;
活性;
【Key words】 Camptotheca acuminata Decne.; Endophytic actinomycetes; Diversity; Activeties;
【Key words】 Camptotheca acuminata Decne.; Endophytic actinomycetes; Diversity; Activeties;
【基金】 NSFC-云南联合基金项目(No.U0932601)
- 【文献出处】 微生物学通报 ,Microbiology China , 编辑部邮箱 ,2010年02期
- 【分类号】S182
- 【被引频次】32
- 【下载频次】509