【摘要】 本文旨在研究临床分离酵母的种类及药物敏感性。采用CHROMagar Candida显色培养基和API20CAUX对我院2008年1～12月分离自临床深部真菌感染患者的1386株酵母进行鉴定,并采用Rosco纸片扩散法分析其对两性霉素B、氟康唑、伊曲康唑、制霉菌素、伏立康唑、5-氟胞嘧啶、克霉唑的药物敏感性,数据用Whonet5.4软件分析。结果显示,分离出的1386株酵母中,白假丝酵母占58.95%,热带假丝酵母占15.95%,光滑假丝酵母占15.15%,克柔假丝酵母占2.74%,其他酵母占7.21%。葡萄牙假丝酵母、近平滑假丝酵母、白假丝酵母、热带假丝酵母、光滑假丝酵母和克柔假丝酵母对氟康唑的敏感率分别为95.45%、94.29%、82.99%、79.19%、68.57%和28.95%;白假丝酵母对5-氟胞嘧啶、两性霉素B、制霉菌素、伏立康唑、氟康唑、伊曲霉唑和克霉唑的敏感率分别为93.76%、93.15%、84.58%、84.09%、82.99%、80.05%和78.09%。结果提示,我院深部真菌感染仍以白假丝酵母为主,其次是热带假丝酵母、光滑假丝酵母;白假丝酵母对5-氟胞嘧啶、两性霉素B的敏感率最高;除克柔假丝酵母外的所有酵母对氟康唑的敏感率都很高。更多还原

【Abstract】 The present paper aims to study clinical yeast isolates and analyze their susceptibility to antifungal drugs. API 20C AUX identification system and CHROMagar Candida medium were used to identify a total of 1 386 strains of yeast collected in our hospital from January to December,2008. The Rosco disk diffusion method was used to test the susceptibility of yeast to amphotericin B,fluconazole,itraconazole,nystatin,voriconazole,5-flucytosine,and clotrimazole. The data were analyzed by Whonet 5.4 software. Of the 1 386 strains collected,Candida albicans (C. albicans) was the most frequent (58.95%),followed by C. tropicalis (15.95%),C. glabrata (15.15%),C. krusei (2.74%),and others (7.21%). Antifungal susceptibility testing results showed that the sensitive rates of C. lusitaniae,C. parapsilosis,C. albicans,C. tropicalis,C. glabrata,and C. krusei to fluconazole were 95.45%,94.29%,82.99%,79.19%,68.57% and 28.95%,respectively. The sensitive rates of C. albicans to 5-flucytosine,amphotericin B,nystatin,voriconazole,fluconazole,itraconazole,and clotrimazole were 93.76%,93.15%,84.58%,84.09%,82.99%,80.05% and 78.09%,respectively. These results suggest that the major pathogen causing deep fungal infection in our hospital is C. albicans,followed by C. tropicalis and C. glabrata. C. albicans was most susceptible to the antifungal effects of 5-flucytosine and amphotericin B. All of the yeast isolates collected,except for C. krusei,were highly sensitive to fluconazole.更多还原