【摘要】 目的检测表没食子儿茶素没食子酸酯(epigallocatechin-3-gallate,EGCG)对体外培养的人胰腺癌细胞系PANC-1增殖的影响。方法缺氧条件下体外培养胰腺癌PANC-1细胞,外源性给予不同剂量的EGCG(10、20、40、60、80、100、160μg/mL)作用细胞不同时间(12、24、48 h),CCK-8法检测不同浓度药物对胰腺癌细胞增殖的影响。结果 EGCG可显著抑制人胰腺癌细胞PANC-1的增殖,其抑制率在药物浓度和时间上呈剂量依赖性和时间依赖性关系;160μg/mL EGCG作用48 h后,PANC-1细胞的生长抑制率高达(91.03±2.32)%。结论 EGCG对体外缺氧培养的人胰腺癌细胞系PANC-1的增殖有显著抑制作用。更多还原

【Abstract】 Objective To investigate the inhibitory effects of epigallocatechin-3-gallate(EGCG) on cell proliferation in human pancreatic carcinoma PANC-1 cell lines.Methods The human pancreatic carcinoma PANC-1 cell lines were incubated under hypoxic condition.Different concentrations of EGCG(10,20,40,60,80,100,160 μg/mL) were applied in cells in vitro for different times(12,24,48 hours).CCK-8 method was used to investigate the effects of drugs on cell proliferation.Results EGCG could inhibit significantly the proliferation of human pancreatic carcinoma PANC-1 cell lines in concentration-dependent and time-dependent manner.There were strong(obvious) inhibition(91.03 ±2.32) % of EGCG(160 μg/mL) on cell proliferation after 48 h treatment.Conclusion EGCG could inhibit significantly the proliferation of human pancreatic carcinoma PANC-1 cell lines in the hypoxic microenvironment.更多还原