【摘要】 15N-螺旋藻是一种重要的同位素示踪剂,应用于生命科学、药物代谢、病理代谢等研究中。由于15N原料十分昂贵,15N-螺旋藻只能由室内小规模生物培养而获得。本工作就室内培养螺旋藻的温度、光照强度、pH等条件进行了探索。结果显示,室温25℃、连续光照、光照强度3 000~4 000 lux、初始pH9.0是室内培养15N-螺旋藻的适宜条件。采用正交设计进行配方优化,优选出培养15N-螺旋藻配方。在优化条件下培养15N-螺旋藻,15N的原料成本投入大幅降低,产品丰度>98%。更多还原

【Abstract】 The spirulina is a phototrophic organism.It was considered as the best of nutritional health product.The nitrogen atoms of spirulina was replaced with 15N stable isotope by the method of biosynthesis.So 15N labeled spirulina is a nutritional health product and stable isotope tracer product.As a tracer,15N labeled spirulina plays an irreplaceable part in bioscience,medicine metabolism and pathology metabolism and so on.15N label spirulina is cultivated only in a small scale because of expensive 15N material.The culture temperature,shine intensity,pH for spirulina were studied.Some feasible conditions were obtained such as 25 ℃,3 000-4 000 lux,initial pH9.0 in the room.15N label spirulin prescription was obtained via orthogonal experiment.The optimize prescription of spirulina was that sodium bicarbonate was 13 g·L-1,15N-urea was 0.2 g·L-1,potassium phosphate dibasic was 0.6 g·L-1,and glucose was 2 g·L-1.The cost of 15N material has a significant reduction.The abundance was more than 98%.更多还原