【摘要】 以山东百部(Stemona Shandongensis D.K.Zang)茎段为外植体,进行离体快繁技术的研究。实验结果表明:启动培养基为MS+6-BA 5.0mg.L-1+NAA0.1mg.L-1+糖30g.L-1+琼脂6.5g.L-1;分化培养基为MS+6-BA 4.0mg.L-1+NAA0.05mg.L-1+糖30g.L-1+琼脂6.5g.L-1;生根培养基为1/2MS+NAA0.1mg.L-1+糖30g.L-1+琼脂6.5g.L-1,通过正交试验也证明了实验结果。更多还原

【Abstract】 Using the stem(Stemona Shandongensis D.K.Zang)buds as explants,this paper study on the art of stem in vitro tissue culture.The results shows that the appropriate initial medium is MS+6-BA 5.0mg·L-1+NAA 0.1mg·L-1+sucrose 30g·L-1+agar 6.5g·L-1;The medium of polarize is MS+6-BA 4.0mg·L-1+NAA 0.05mg·L-1+sucrose 30g·L-1+agar 6.5g·L-1;The medium producing root is 1/2MS+NAA 0.1mg·L-1+sucrose 30g·L-1+agar 6.5g·L-1.The tested by orthogonal also proved the results.更多还原