【摘要】 建立大鼠血浆和脑中Z-槀苯内酯(LIG)浓度测定的高效液相色谱法。采用Agilent Hypersil ODS C18色谱柱(150mm×4.6mm,5μm),流动相为甲醇-5%异丙醇水溶液(60:40,v/v),流速为1.0mL/min,检测波长为280nm。血浆与脑中槀苯内酯浓度线性检测范围分别为93.75~3750ng/m(r=0.9999)和93.75~3750ng/g(r=0.9997),日内及日间精密度RSD<10%。本法适用于大鼠口服LIG后血浆及脑中药物浓度的研究。更多还原

【Abstract】 A high performance liquid chromatographic(HPLC)method was developed and validated for the determination of Z-ligustilide(LIG)concentrations in rat plasma and brain.The analysis used an Agilent Hypersil ODS C18 column(5 μm,150 mm × 4.6 mm i.d.)and methanol-5% aqueous isopronaol(60:40,v/v)as the mobile phase(flow rate = 1.0 mL/min).The detection wavelength was set at 280 nm.The calibration curves were linear over the range of 93.75-3750 ng/mL with a correlation coefficient of 0.9999 in plasma and 93.75-3750 ng/g with a correlation coefficient of 0.9997 in brain,respectively.The RSD of intra-and inter-day assay variations were all less than 10% at the concentration range from 93.75 to 3750 ng/mL(g).The established assay method was successfully applied to the study of the plasma and brain concentrations of LIG in rats following oral administration.更多还原