【摘要】 目的:研究GPⅡb/Ⅲa受体拮抗剂替罗非班对猪急性心肌梗死(AMI)再灌注后细胞因子的影响。方法:将19只猪随机分为对照组(n=10)和替罗非班组(n=9)。通过介入球囊封闭冠状动脉的方法制作AMI再灌注模型。再灌注后1、24、48及72h进行DE-MSCT检查,分别于AMI前30min、AMI后30min、再灌注后10min、2h、24h、48h及72h取静脉血,应用酶联免疫吸附(ELISA)法测定血清白细胞介素(IL)-6、IL-10、缺氧诱导因子(HIF)-1α浓度。结果:(1)替罗非班组梗死心肌体积和无复流体积在再灌注后明显小于对照组(P<0.05)。(2)替罗非班组和对照组中,AMI后30min、再灌注后10min～72h各时间点的血清IL-6和IL-10水平较AMI前升高;再灌注后,替罗非班组血清IL-6水平均显著低于对照组(P<0.05),而血清IL-10水平高于对照组(P<0.05)。(3)再灌注后,血清HIF-1α较AMI前明显升高,再灌注后24、48及72h替罗非班组显著低于对照组(P<0.05)。结论:AMI再灌注后缺血心肌发生炎症反应;替罗非班能够减轻梗死再灌注后的炎症反应,进而减少心肌进一步损伤。更多还原

【Abstract】 Objective:To investigate the effects of tirofiban on cytokines in pigs with myocardial infarction reperfusion.Methods:Nineteen Chinese mini pigs were randomized into control group(n = 10) and tirofiban-treating group(n = 9).The model of acute myocardial infarction(AMI) was induced by the method of percutaneous transluminal coronary angioplasty(PTCA).All the successful models accepted DE-MSCT examinations at 1 h,24 h,48 h,and 72 h after reperfusion.All pigs were taken blood samples at seven different points:30min before AMI,30 min after AMI,10 min,2 h,24 h,48 h and 72 h after reperfusion.The serum levels of interleukin(IL)-6,IL-10 and hypoxia inducible factor(HIF)-1α were measured by enzyme linked immunosorbent assay(ELISA).Results:(1)The percentages of infarct myocardium volume and no-reflow volume after reperfusion were significantly reduced in tirofiban-treating group compared with those of control group(P < 0.05).(2)Compared with the baseline,the levels of serum IL-6 and IL-10 were increased at 30 min after AMI and 10 min～72 h after reperfusion in tirofiban-treating group and control group.In tirofiban-treating group,the level of serum IL-6 was significantly lower than that in control group(P < 0.05),while the level of serum IL-10 was higher than that in control group(P < 0.05).(3)After reperfusion,the serum level of HIF-1α was increased compared with the baseline in tirofiban-treating group and control group.The serum level of HIF-1α was significantly lower in tirofiban-treating group than that of control group after 24 h,48 h and 72 h reperfusion(P < 0.05).Conclusion:Inflammation reaction occurs in ischemic myocardium after AMI and reperfusion.Tirofiban is effective in anti-inflammation and reducing the progress of myocardial necrosis.更多还原