【摘要】 目的通过对肺腺癌和肺鳞状细胞癌中转化生长因子-βⅡ型受体(TGF-βRⅡ)、核转录因子-κB(NF-κB)的表达及肿瘤相关性巨噬细胞(TAMs)计数的研究,探讨三者与肺腺癌和肺鳞状细胞癌的关系。方法应用免疫组织化学技术检测TGF-βRⅡ蛋白、NF-κB蛋白及肿瘤相关性巨噬细胞在肺腺癌和肺鳞状细胞癌组织中的表达,并与癌旁正常肺组织中的表达进行比较。结果①TGF-βRⅡ蛋白的表达在肿瘤组织中明显降低(P<0.01),其表达下调与肺癌的组织学类型、淋巴结转移、临床分期相关(均P<0.05)。②NF-κB蛋白的表达在肿瘤组织中明显增强(P<0.01),其表达增强与肿瘤大小、淋巴结转移、临床分期相关(P<0.01,P<0.05)。③TAMs在肿瘤间质内浸润数量明显增多(P<0.01),并与肺癌的组织学类型、肿瘤大小、淋巴结转移、临床分期密切相关(P<0.05,P<0.01)。④TGF-βRⅡ蛋白与NF-κB蛋白的表达呈负相关(P<0.01),NF-κB蛋白的表达与TAMs的浸润数量呈正相关(P<0.01)。结论 TGF-βRⅡ蛋白表达水平降低可能促进肺癌的发生、浸润和转移,对肺腺癌发生或进展可能影响更大;NF-κB可能通过下调TGF-βRⅡ的表达、吸引肿瘤相关性巨噬细胞至肿瘤间质而促进肿瘤的发生、发展,且这种作用在非小细胞肺癌中具有较普遍的意义。更多还原

【Abstract】 Objective To investigate the expression of TGF-β RⅡ,NF-κB and the amount of tumor-associated macrophages(TAMs)in lung adenocarcinoma and lung squamous cell carcinoma and their correlation.Methods The expression of TGF-βRⅡ,NF-κB and TAMs was detected by immunohistochemistry in lung adenocarcinoma group,lung squamous cell carcinoma group and control group.Results ①As compared with the control group,the expression of TGF-βRⅡ was decreased in lung adenocarcinoma group and squamous cell carcinoma group(P<0.01),and it was correlated with histological type,lymph node metastasis and clinical stage(P<0.05);②As compared with the control group,the expression of NF-κB was increased in lung adenocarcinoma group and squamous cell carcinoma group(P<0.01),and it was correlated with tumor size,lymph node metastasis and clinical stage(P<0.01,P<0.05);③As compared with the control group,the number of TAMs was increased in tumor groups(P<0.01),and it was correlated with histological type,tumor size,lymph node metastasis and clinical stage(P<0.05,P<0.01);④The expression of TGF-βRⅡ was negatively correlated with that of NF-κB p65 protein(P<0.01).The number of TAMs was positively correlated with the expression of NF-κB p65 protein(P<0.01).Conclusion Low expression of TGF-βRⅡ may be related with the tumorigenesis of lung and carcinomatous growth,invasion and metastasis.This effect may be more significant in lung adenocarcinoma than in squamous cell carcinoma.NF-κB probably promotes tumorigenesis through down-regulating the expression of TGF-βRⅡ and attracting TAMs into tumor stroma.There is a more general significance of the effect in non-small cell lung cancer.更多还原