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苯酚-硫酸比色法测定红毛五加皮多糖的含量

Determination of Polysaccharides in Cortex acanthopanacis giraldii by Phenol-sulfuric acid Colorimetry

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【作者】 钟世红卫莹芳古锐黄小雪谢武超杨湘

【Author】 ZHONG Shi-hong1,2,WEI Ying-fang2,GU Rui2,HUANG Xiao-xue1,XIE Wu-chao1,YANG Xiang2(1.Pharmacological School,Chengdu Medical College,Chengdu 610081,China;2.Chengdu University of TCM,Chengdu 611131,China)

【机构】 成都医学院药学院成都中医药大学

【摘要】 目的建立红毛五加皮多糖的含量测定方法,对不同产地样品进行测定。方法采用苯酚-硫酸比色法。结果葡萄糖的线性范围为24.05~54.11μg/ml,平均加样回收率为97.80%,RSD为1.59%(n=6)。红毛五加皮的多糖含量为6.83%~24.50%;木心的多糖含量为5.78%~9.21%。结论该方法快速、准确,可为红毛五加药材的质量控制提供参考。建议红毛五加皮多糖含量不得低于10.9%,木心具有一定利用价值。

【Abstract】 Objective To determine the content of polysaccharides in cortex acanthopanacis giraldii from different regions.Methods Phenol-sulfuric acid colorimetry was used to determine the content of polysaccharides.Results The linear range of glucose was 24.05~54.11 μg / ml.The average recovery was 97.80%,and RSD was 1.59%(n=6).The polysaccharides content of bark was within the range of 6.83%~24.50% and that of xylem was within the range of 5.78%~9.21%.Conclusion The method is rapid,accurate and can be used for the quantity control of A.Giraldii Harms.The polysaccharides content of bark should not be lower than 10.9%.The xylem is valuable in some degree.

【基金】 国家自然科学基金青年基金(No.30701087)
  • 【文献出处】 时珍国医国药 ,Lishizhen Medicine and Materia Medica Research , 编辑部邮箱 ,2010年02期
  • 【分类号】R284.1
  • 【被引频次】37
  • 【下载频次】564
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