【摘要】 目的:探讨以jetPEI-RGD为转染载体的125I-(αV)ASODN投递在体外对HepG2细胞侵袭力的影响。方法:125I标记整合素αV亚基的ASODN,以聚乙烯亚胺衍生物jetPEI-RGD为载体,通过受体介导的转染方式进入HepG2细胞,利用Boyden小室侵袭模型评估复合物对HepG2细胞侵袭力的影响,并计算实验组和各对照组的抑制率。结果:jetPEI-RGD/125I-(αV)ASODN组、jetPEI-RGD/(αV)ASODN组、jetPEI-RGD组、125I-(αV)ASODN组、(αV)ASODN组和Na125I组的抑制率分别为(52.60±4.11)%、(39.73±3.40)%、(30.05±5.19)%、(14.14±2.94)%、(12.79±2.68)%和(0.91±2.91)%,相对于其他各实验对照组,jetPEI-RGD/125I-(αV)ASODN组减低了HepG2细胞的侵袭能力(P<0.01)。结论:以jetPEI-RGD为载体投递125I-(αv)ASODN能有效抑制HepG2细胞的侵袭力。更多还原

【Abstract】 Objective To evaluate the effects of 125I-(αV) antisense oligodeoxynucleotides (ASODN) delivered by jetPEI-RGD on the invasion ability of human hepatic carcinoma cell line (Hep G2)cultured in vitro. Methods αV ASODN labeled with 125I was transfected into HepG2 cells with polyethyleneimine derivatives jetPEI-RGD in a receptor mediated manner. Cell invasion was evaluated by using a Boyden chamber assay, and the inhibition rates of different treatments were calculated. Results The inhibition rates of jetPEI-RGD / 125I-(αV) ASODN、jetPEI-RGD / (αV)ASODN、jetPEI-RGD、125I-(αV)ASODN、 (αV)ASODN and Na125I on the cell invasion of Hep G2 cells were (52.60 ± 4.11)%、(39.73 ± 3.40)%、(30.05 ± 5.19)%、(14.14 ± 2.94)%、(12.79 ± 2.68)% and(0.91 ± 2.91)%,respectively. In contrast to the other experimental groups and control group, jetPEI-RGD / 125I-(αV)ASODN treatment significantly reduced the invasive capacity of Hep G2 cells (P < 0.01). Conclusion 125I-(αV)ASODN delivered by jetPEI-RGD effectively reduced the invasion ability of Hep G2 cells.更多还原